Western Blot (WB)
(HSD3B1 Antibody (N-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the HSD3B1 antibody detected the HSD3B1 protein (arrow).)
Rabbit anti-Human HSD3B1 Polyclonal Antibody | anti-HSD3B1 antibody
HSD3B1, NT (HSD3B1, 3BH, HSDB3A, 3 beta-hydroxysteroid dehydrogenase/Delta 5->4-isomerase type 1, 3 beta-hydroxysteroid dehydrogenase/Delta 5->4-isomerase type I, Trophoblast antigen FDO161G, 3-beta-hydroxy-Delta(5)-steroid dehydrogenase, 3-beta-hydroxy-5
Gene Names
HSD3B1; I; HSD3B; HSDB3; HSDB3A; SDR11E1; 3BETAHSD
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
HSD3B1; Polyclonal Antibody; NT (HSD3B1; 3BH; HSDB3A; 3 beta-hydroxysteroid dehydrogenase/Delta 5->4-isomerase type 1; 3 beta-hydroxysteroid dehydrogenase/Delta 5->4-isomerase type I; Trophoblast antigen FDO161G; 3-beta-hydroxy-Delta(5)-steroid dehydrogenase; 3-beta-hydroxy-5; 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1; 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I; 3-beta-hydroxy-5-ene steroid dehydrogenase; Progesterone reductase; Steroid Delta-isomerase; Delta-5-3-ketosteroid isomerase; anti-HSD3B1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Applicable Applications for anti-HSD3B1 antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
HSD3B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 25-55 amino acids from the N-terminal region of human HSD3B1.
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(HSD3B1 Antibody (N-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the HSD3B1 antibody detected the HSD3B1 protein (arrow).)
Western Blot (WB)
(HSD3B1 Antibody (N-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the HSD3B1 antibody detected the HSD3B1 protein (arrow).)
Western Blot (WB)
(Western blot analysis of HSD3B1 (arrow) using rabbit polyclonal HSD3B1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HSD3B1 gene.)
Western Blot (WB)
(Western blot analysis of HSD3B1 (arrow) using rabbit polyclonal HSD3B1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HSD3B1 gene.)
Related Product Information for anti-HSD3B1 antibody
3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. Efficiently catalyzes the transformation of pregnenolone to progesterone, 17-alpha-hydroxypregnenolone to 17-alpha-hydroxyprogesterone, DHEA to 4-androstenedione, dihydrotestosterone to 5-alpha-androstane-3 beta,17 beta-diol, dehydroepiandrosterone to androstenedione and 5-alpha-androstan-3 beta,17 beta-diol to 5-alpha-dihydrotestosterone.
Product Categories/Family for anti-HSD3B1 antibody
References
Canzian, F., et al. Hum. Mol. Genet. 19(19):3873-3884(2010). Shimodaira, M., et al. Eur. J. Endocrinol. 163(4):671-680(2010). Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010). Thomas, J.L., et al. J. Steroid Biochem. Mol. Biol. 120 (4-5), 192-199 (2010).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
42,252 Da
NCBI Official Full Name
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
NCBI Official Synonym Full Names
hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1
NCBI Official Symbol
HSD3B1
NCBI Official Synonym Symbols
I; HSD3B; HSDB3; HSDB3A; SDR11E1; 3BETAHSD
NCBI Protein Information
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
UniProt Protein Name
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
Protein Family
UniProt Gene Name
HSD3B1
UniProt Synonym Gene Names
3BH; HSDB3A; 3-beta-HSD I
UniProt Entry Name
3BHS1_HUMAN
Research Articles on HSD3B1
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Product Notes
The HSD3B1 hsd3b1 (Catalog #AAA6308521) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HSD3B1, NT (HSD3B1, 3BH, HSDB3A, 3 beta-hydroxysteroid dehydrogenase/Delta 5->4-isomerase type 1, 3 beta-hydroxysteroid dehydrogenase/Delta 5->4-isomerase type I, Trophoblast antigen FDO161G, 3-beta-hydroxy-Delta(5)-steroid dehydrogenase, 3-beta-hydroxy-5 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HSD3B1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the HSD3B1 hsd3b1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSD3B1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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