Rabbit anti-Human HSD17B12 Polyclonal Antibody | anti-HSD17B12 antibody

HSD17B12, ID (HSD17B12, Estradiol 17-beta-dehydrogenase 12, 17-beta-hydroxysteroid dehydrogenase 12, 3-ketoacyl-CoA reductase) (Azide free) (HRP)

Cat. Num. AAA6308478

• Gene Names: HSD17B12; KAR; SDR12C1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: HSD17B12; Polyclonal Antibody; ID (HSD17B12; Estradiol 17-beta-dehydrogenase 12; 17-beta-hydroxysteroid dehydrogenase 12; 3-ketoacyl-CoA reductase) (Azide free) (HRP); 3-ketoacyl-CoA reductase; anti-HSD17B12 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HSD17B12.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-HSD17B12 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 126-155 in the central region of human HSD17B12, UniProt Accession #Q53GQ0. Species sequence homology: monkey
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of A2058 cell line lysates (35ug/lane) usingMBS6013293. HSD17B12 (arrow) was detected usingMBS6013293.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma usingMBS6013293, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry.)

Testing Data

()

Product Categories/Family for anti-HSD17B12 antibody

Antibodies; Enzymes; Dehydrogenase

References

Plourde, M. J. Steroid Biochem. Mol. Biol. 116 (3-5), 134-153 (2009). Nagasaki, S. Mol. Cell. Endocrinol. 307 (1-2), 163-168 (2009). Bellemare, V. J. Steroid Biochem. Mol. Biol. 114 (3-5), 129-134 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 7705855
• NCBI GeneID: 51144
• NCBI Accession #: NP_057226.1
• NCBI GenBank Nucleotide #: NM_016142.2
• UniProt Accession #: Q53GQ0; Q96JU2; Q9Y6G8; A8K9B0; D3DR23
• Molecular Weight: 34,324 Da
• NCBI Official Full Name: estradiol 17-beta-dehydrogenase 12
• NCBI Official Synonym Full Names: hydroxysteroid (17-beta) dehydrogenase 12
• NCBI Official Symbol: HSD17B12
• NCBI Official Synonym Symbols: KAR; SDR12C1
• NCBI Protein Information: estradiol 17-beta-dehydrogenase 12; 17-beta-HSD 12; 17beta-HSD type 12; 3-ketoacyl-CoA reductase; steroid dehydrogenase homolog; 17-beta-hydroxysteroid dehydrogenase 12; short chain dehydrogenase/reductase family 12C, member 1
• UniProt Protein Name: Estradiol 17-beta-dehydrogenase 12
• Protein Family: Very-long-chain 3-oxoacyl-CoA reductase
• UniProt Gene Name: HSD17B12
• UniProt Synonym Gene Names: 17-beta-HSD 12; KAR
• UniProt Entry Name: DHB12_HUMAN

NCBI Description

This gene encodes a very important 17beta-hydroxysteroid dehydrogenase (17beta-HSD) that converts estrone into estradiol in ovarian tissue. This enzyme is also involved in fatty acid elongation. [provided by RefSeq, Oct 2011]

Uniprot Description

HSD17B12: Catalyzes the transformation of estrone (E1) into estradiol (E2), suggesting a central role in estrogen formation. Its strong expression in ovary and mammary gland suggest that it may constitute the major enzyme responsible for the conversion of E1 to E2 in women. Also has 3-ketoacyl-CoA reductase activity, reducing both long chain 3-ketoacyl-CoAs and long chain fatty acyl-CoAs, suggesting a role in long fatty acid elongation. Belongs to the short-chain dehydrogenases/reductases (SDR) family. 17-beta-HSD 3 subfamily.

Protein type: Membrane protein, integral; Oxidoreductase; Lipid Metabolism - androgen and estrogen; EC 1.1.1.62; Lipid Metabolism - unsaturated fatty acid biosynthesis; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: extracellular matrix; endoplasmic reticulum membrane; integral to membrane

Molecular Function: heparin binding; collagen binding; estradiol 17-beta-dehydrogenase activity; protein binding; fibronectin binding

Biological Process: extracellular matrix organization and biogenesis; triacylglycerol biosynthetic process; cellular lipid metabolic process; estrogen biosynthetic process; fatty acid biosynthetic process