Rabbit anti-Human HSD11B2 Polyclonal Antibody | anti-Hsd11b2 antibody

HSD11B2 (HSD11K, Corticosteroid 11-beta-dehydrogenase Isozyme 2, 11-beta-hydroxysteroid Dehydrogenase Type 2, 11-beta-hydroxysteroid Dehydrogenase Type II, NAD-dependent 11-beta-hydroxysteroid Dehydrogenase)

Cat. Num. AAA6005222

• Gene Names: Hsd11b2; 11HSD2
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: HSD11B2; Polyclonal Antibody; HSD11B2 (HSD11K; Corticosteroid 11-beta-dehydrogenase Isozyme 2; 11-beta-hydroxysteroid Dehydrogenase Type 2; 11-beta-hydroxysteroid Dehydrogenase Type II; NAD-dependent 11-beta-hydroxysteroid Dehydrogenase); Anti -HSD11B2 (HSD11K; anti-Hsd11b2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HSD11B2.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Sequence: MERWPWPSGGAWLLVAARALLQLLRSDLRLGRPLLAALALLAALDWLCQRLLPPPAALAVLAAAGWIALSRLARPQRLPVATRAVLITGCDSGFGKETAKKLDSMGFTVLATVLELNSPGAIELRTCCSPRLRLLQMDLTKPGDISRVLEFTKAHTTSTGLWGLVNNAGHNEVVADAELSPVATFRSCMEVNFFGALELTKGLLPLLRSSRGRIVTVGSPAGDMPYPCLGAYGTSKAAVALLMDTFSCELLPWGVKVSIIQPGCFKTESVRNVGQWEKRKQLLLANLPQELLQAYGKDYIEHLHGQFLHSLRLAMSDLTPVVDAITDALLAARPRRRYYPGQGLGLMYFTHYYLPEGLRRRFLQAFFISHCLPRALQPGQPGTTPPQDAAQDPNLSPGPSPAVAR

• Applicable Applications for anti-Hsd11b2 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.
• Immunogen: Full length human HSD11B2, aa1-405 (AAH64536.1).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(HSD11B2 rabbit polyclonal antibody. Western Blot analysis of HSD11B2 expression in human placenta.)

Western Blot (WB)

(Western Blot analysis of HSD11B2 expression in transfected 293T cell line by HSD11B2 polyclonal antibody. Lane 1: HSD11B2 transfected lysate (44.1kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-Hsd11b2 antibody

There are at least two isozymes of the corticosteroid 11-beta-dehydrogenase, a microsomal enzyme complex responsible for the interconversion of cortisol and cortisone. The type I isozyme has both 11-beta-dehydrogenase (cortisol to cortisone) and 11-oxoreductase (cortisone to cortisol) activities. The type II isozyme, encoded by this gene, has only 11-beta-dehydrogenase activity. In aldosterone-selective epithelial tissues such as the kidney, the type II isozyme catalyzes the glucocorticoid cortisol to the inactive metabolite cortisone, thus preventing illicit activation of the mineralocorticoid receptor. In tissues that do not express the mineralocorticoid receptor, such as the placenta and testis, it protects cells from the growth-inhibiting and/or pro-apoptotic effects of cortisol, particularly during embryonic development. Mutations in this gene cause the syndrome of apparent mineralocorticoid excess and hypertension.

Product Categories/Family for anti-Hsd11b2 antibody

Antibodies; Abs to Enzymes; Dehydrogenase

NCBI and Uniprot Product Information

• NCBI GI #: 42490824
• NCBI GeneID: 15484
• UniProt Accession #: P51661; Q91WK3
• Molecular Weight: 42,187 Da
• NCBI Official Full Name: Hsd11b2 protein
• NCBI Official Synonym Full Names: hydroxysteroid 11-beta dehydrogenase 2
• NCBI Official Symbol: Hsd11b2
• NCBI Official Synonym Symbols: 11HSD2
• NCBI Protein Information: corticosteroid 11-beta-dehydrogenase isozyme 2; 11-DH2; 11-beta-HSD2; 11(beta)-HSD2; 11-beta-hydroxysteroid dehydrogenase type 2; NAD-dependent 11-beta-hydroxysteroid dehydrogenase
• UniProt Protein Name: Corticosteroid 11-beta-dehydrogenase isozyme 2
• Protein Family: Corticosteroid 11-beta-dehydrogenase isozyme
• UniProt Gene Name: Hsd11b2
• UniProt Synonym Gene Names: Hsd11k; 11-DH2
• UniProt Entry Name: DHI2_MOUSE

Uniprot Description

HSD11B2: Catalyzes the conversion of cortisol to the inactive metabolite cortisone. Modulates intracellular glucocorticoid levels, thus protecting the nonselective mineralocorticoid receptor from occupation by glucocorticoids. Defects in HSD11B2 are the cause of apparent mineralocorticoid excess (AME). An autosomal recessive form of low-renin hypertension. It is usually diagnosed within the first years of life and is characterized by polyuria and polydipsia, failure to thrive, hypernatremia, severe hypertension with low renin and aldosterone levels, profound hypokalemia with metabolic alkalosis, and most often nephrocalcinosis. Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Protein type: Lipid Metabolism - androgen and estrogen; EC 1.1.1.-; Lipid Metabolism - C21-steroid hormone; Oxidoreductase

Cellular Component: intracellular membrane-bound organelle; endoplasmic reticulum; cytoplasm

Molecular Function: mevaldate reductase activity; (R)-2-hydroxyglutarate dehydrogenase activity; phenylcoumaran benzylic ether reductase activity; aldo-keto reductase activity; arabinose reductase activity; isocitrate dehydrogenase activity; oxidoreductase activity; C-3 sterol dehydrogenase (C-4 sterol decarboxylase) activity; steroid binding; epoxide dehydrogenase activity; (R)-2-hydroxyisocaproate dehydrogenase activity; steroid dehydrogenase activity; 3-ketoglucose-reductase activity; 11-beta-hydroxysteroid dehydrogenase activity; steroid dehydrogenase activity, acting on the CH-CH group of donors; D-arabinitol dehydrogenase (NADP+) activity; 2-hydroxytetrahydrofuran dehydrogenase activity; 5-exo-hydroxycamphor dehydrogenase activity; NAD binding; acetoin dehydrogenase activity; steroid dehydrogenase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; xylose reductase activity; gluconate dehydrogenase activity

Biological Process: glucocorticoid metabolic process; metabolic process; aldosterone mediated regulation of blood volume; female pregnancy