Rabbit anti-Human Hrk Polyclonal Antibody | anti-Hrk antibody

Hrk, BH3 Domain (Activator of Apoptosis Harakiri, Neuronal Death Protein DP5, BH3-interacting Domain-containing Protein 3, BID3) (PE)

Cat. Num. AAA6481172

• Gene Names: HRK; DP5; HARAKIRI
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: Hrk; Polyclonal Antibody; BH3 Domain (Activator of Apoptosis Harakiri; Neuronal Death Protein DP5; BH3-interacting Domain-containing Protein 3; BID3) (PE); anti-Hrk antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Hrk.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-Hrk antibody: Immunohistochemistry (IHC), FLISA
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from a portion within aa30-50 of human Hrk (KLH).
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Hrk-BH3 western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the Hrk antibody detected the Hrk protein (arrow).)

Immunohistochemistry (IHC)

(Hrk BH3 Domain Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Hrk BH3 Domain Antibody for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-Hrk antibody

Antibodies; Apoptosis

References

1. Wakabayashi, T., et al., Neurosci. Lett. 318(2):77-80 (2002). 2. Inohara, N., et al., EMBO J. 16(7):1686-1694 (1997).

NCBI and Uniprot Product Information

• NCBI GI #: 4504493
• NCBI GeneID: 8739
• NCBI Accession #: NP_003797
• NCBI GenBank Nucleotide #: NM_003806.2
• UniProt Accession #: O00198
• Molecular Weight: 9,884 Da
• NCBI Official Full Name: activator of apoptosis harakiri
• NCBI Official Synonym Full Names: harakiri, BCL2 interacting protein
• NCBI Official Symbol: HRK
• NCBI Official Synonym Symbols: DP5; HARAKIRI
• NCBI Protein Information: activator of apoptosis harakiri; death protein 5; BCL2-interacting protein; activator of apoptosis Hrk; neuronal death protein DP5; BH3-interacting domain-containing protein 3; harakiri, BCL2 interacting protein (contains only BH3 domain)
• UniProt Protein Name: Activator of apoptosis harakiri
• Protein Family: Activator of apoptosis harakiri
• UniProt Gene Name: HRK
• UniProt Synonym Gene Names: BID3
• UniProt Entry Name: HRK_HUMAN

NCBI Description

This gene encodes a member of the BCL-2 protein family. Members of this family are involved in activating or inhibiting apoptosis. The encoded protein localizes to intracellular membranes. This protein promotes apoptosis by interacting with the apoptotic inhibitors BCL-2 and BCL-X(L) via its BH3 domain. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Oct 2012]

Uniprot Description

HRK: Promotes apoptosis.

Protein type: Membrane protein, integral; Apoptosis

Chromosomal Location of Human Ortholog: 12q24.22

Cellular Component: mitochondrion; integral to membrane

Molecular Function: protein binding

Biological Process: apoptosis; positive regulation of apoptosis; positive regulation of protein homooligomerization