Mouse hIST1 Monoclonal Antibody | anti-hIST1 antibody

Anti-hIST1 Antibody

Cat. Num. AAA415188

• Gene Names: IST1; OLC1
• Applications: ELISA, Western Blot, Western Blot
• Purity: Affinity purified
• Synonyms: hIST1; Monoclonal Antibody; Anti-hIST1 Antibody; anti-hIST1 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: Anti-hIST1 recognizes hIST1 expression in H520 whole cell lysate.
• Purity/Purification: Affinity purified
• Form/Format: Liquid
• Concentration: 100ug/100ul (varies by lot)
• Sequence Length: 2377

• Applicable Applications for anti-hIST1 antibody: ELISA (EIA), Western Blot (WB-Re), Western Blot (WB-Ce)
• Application Notes: WB: 1:1000 analysis of hIST1 expression in H520 whole cell lysate with Anti-hIST1
• Raised In: Purified mouse monoclonal antibody generated using E.coli expressed recombinant protein hIST1 as immunogen.
• Storage Buffer: citrate-tris-HCl buffer pH7.0 with 0.02% Proclin 300.
• Immunogen: Recombinant protein corresponding to full length hIST1.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

References

None

NCBI and Uniprot Product Information

• NCBI GI #: 41281488
• NCBI GeneID: 9798
• NCBI Accession #: NP_055576.2
• NCBI GenBank Nucleotide #: NM_014761.2
• Molecular Weight: 23,089 Da
• NCBI Official Full Name: Homo sapiens increased sodium tolerance 1 homolog (yeast) (IST1), mRNA
• NCBI Official Synonym Full Names: increased sodium tolerance 1 homolog (yeast)
• NCBI Official Symbol: IST1
• NCBI Official Synonym Symbols: OLC1
• NCBI Protein Information: IST1 homolog; overexpressed in lung cancer 1; putative MAPK-activating protein PM28
• UniProt Protein Name: IST1 homolog
• UniProt Gene Name: IST1
• UniProt Entry Name: IST1_HUMAN

NCBI Description

This gene encodes a protein with MIT-interacting motifs that interacts with components of endosomal sorting complexes required for transport (ESCRT). ESCRT functions in vesicle budding, such as that which occurs during membrane abscission in cytokinesis. There is a pseudogene for this gene on chromosome 19. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012]

Uniprot Description

IST1: Proposed to be involved in specific functions of the ESCRT machinery. Is required for efficient abscission during cytokinesis, but not for HIV-1 budding. The involvement in the MVB pathway is not established. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells. Belongs to the IST1 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Vesicle

Chromosomal Location of Human Ortholog: 16q22.2

Cellular Component: centrosome; cytoplasmic membrane-bound vesicle; ER-Golgi intermediate compartment; midbody; cytosol

Molecular Function: protein domain specific binding; protein binding; protein complex binding

Biological Process: release of virus from host; establishment of protein localization; protein localization; cell division; positive regulation of proteolysis; abscission; cytokinesis; viral capsid re-envelopment; positive regulation of collateral sprouting