Rabbit HPD Polyclonal Antibody | anti-HPD antibody

HPD Polyclonal Antibody

Cat. Num. AAA9126081

• Gene Names: HPD; PPD; 4HPPD; GLOD3; 4-HPPD; HPPDASE
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purification
• Synonyms: HPD; Polyclonal Antibody; HPD Polyclonal Antibody; PPD; 4HPPD; GLOD3; 4-HPPD; HPPDASE; anti-HPD antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 393

• Applicable Applications for anti-HPD antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50 - 1:200
• Species: Human
• Immunogen: Recombinant Protein
• Immunogen: Recombinant protein of human HPD
• Calculated Molecular Weight: 45kDa
• Preparation and Storage: Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.; Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using HPD antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 10s.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat kidney using HPD antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse kidney using HPD antibody at dilution of 1:100 (40x lens).)

Immunofluorescence (IF)

(Immunofluorescence analysis of U2OS cells using HPD antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-HPD antibody

The protein encoded by this gene is an enzyme in the catabolic pathway of tyrosine. The encoded protein catalyzes the conversion of 4-hydroxyphenylpyruvate to homogentisate. Defects in this gene are a cause of tyrosinemia type 3 (TYRO3) and hawkinsinuria (HAWK). Two transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-HPD antibody

Polyclonal

NCBI and Uniprot Product Information

• NCBI GI #: 417144
• NCBI GeneID: 3242
• UniProt Accession #: P32754; Q13234; A8K461; B3KQ63
• Molecular Weight: 393
• NCBI Official Full Name: 4-hydroxyphenylpyruvate dioxygenase
• NCBI Official Synonym Full Names: 4-hydroxyphenylpyruvate dioxygenase
• NCBI Official Symbol: HPD
• NCBI Official Synonym Symbols: PPD; 4HPPD; GLOD3; 4-HPPD; HPPDASE
• NCBI Protein Information: 4-hydroxyphenylpyruvate dioxygenase; glyoxalase domain containing 3; 4-hydroxyphenylpyruvic acid oxidase
• UniProt Protein Name: 4-hydroxyphenylpyruvate dioxygenase
• UniProt Gene Name: HPD
• UniProt Synonym Gene Names: PPD; 4HPPD; HPD; HPPDase
• UniProt Entry Name: HPPD_HUMAN

NCBI Description

The protein encoded by this gene is an enzyme in the catabolic pathway of tyrosine. The encoded protein catalyzes the conversion of 4-hydroxyphenylpyruvate to homogentisate. Defects in this gene are a cause of tyrosinemia type 3 (TYRO3) and hawkinsinuria (HAWK). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2010]

Uniprot Description

HPD: Key enzyme in the degradation of tyrosine. Defects in HPD are the cause of tyrosinemia type 3 (TYRO3). TYRO3 is an inborn error of metabolism characterized by elevations of tyrosine in the blood and urine, seizures and mild mental retardation. Defects in HPD are a cause of hawkinsinuria (HAWK). HAWK is an inborn error of tyrosine metabolism characterized by failure to thrive, persistent metabolic acidosis, fine and sparse hair, and excretion of the unusual cyclic amino acid metabolite, hawkinsin, in the urine. Belongs to the 4HPPD family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - tyrosine; Amino Acid Metabolism - phenylalanine; Oxidoreductase; EC 1.13.11.27; Cofactor and Vitamin Metabolism - ubiquinone and other terpenoid-quinone biosynthesis

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: cytosol

Molecular Function: 4-hydroxyphenylpyruvate dioxygenase activity; metal ion binding

Biological Process: L-phenylalanine catabolic process; tyrosine catabolic process

Disease: Hawkinsinuria; Tyrosinemia, Type Iii