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SDS-Page

ST8SIA1 recombinant protein

ST8SIA1 Protein, Human, Recombinant (His Tag)

Gene Names
ST8SIA1; GD3S; SIAT8; SIAT8A; SIAT8-A; ST8SiaI
Purity
>95% as determined by SDS-PAGE
Synonyms
ST8SIA1; ST8SIA1 Protein; Human; Recombinant (His Tag); Human ST8SIA1/GD3S Protein (His Tag); GD3S Protein; SIAT8 Protein; SIAT8-A Protein; SIAT8A Protein; ST8SiaI Protein; ST8 alpha-N-acetyl-neuraminide alpha-2; 8-sialyltransferase 1; ST8SIA1 recombinant protein
Ordering
For Research Use Only!
Host
HEK293 Cells
Purity/Purification
>95% as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Sequence
Thr49-Ser356
Species
Human
Endotoxin
<1.0EU per ug protein as determined by the LAL method.
Predicted N Terminal
Tyr 49
Tag
C-His
Protein Construction
A DNA sequence encoding the human ST8SIA1 (NP_003025.1)(Tyr49-Ser356) was expressed with a polyhistidine tag at the C-terminus.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.

SDS-Page

SDS-Page

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
40,519 Da
NCBI Official Full Name
alpha-N-acetylneuraminide alpha-2,8-sialyltransferase
NCBI Official Synonym Full Names
ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1
NCBI Official Symbol
ST8SIA1
NCBI Official Synonym Symbols
GD3S; SIAT8; SIAT8A; SIAT8-A; ST8SiaI
NCBI Protein Information
alpha-N-acetylneuraminide alpha-2,8-sialyltransferase; ganglioside GD3 synthase; ganglioside GT3 synthase; sialytransferase St8Sia I; sialyltransferase St8Sia I; alpha-2,8-sialyltransferase 8A; disialoganglioside (GD3) synthase; ganglioside-specific alpha
UniProt Protein Name
Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase
UniProt Gene Name
ST8SIA1
UniProt Synonym Gene Names
SIAT8; SIAT8A; SIAT8-A; ST8SiaI
UniProt Entry Name
SIA8A_HUMAN

NCBI Description

Gangliosides are membrane-bound glycosphingolipids containing sialic acid. Ganglioside GD3 is known to be important for cell adhesion and growth of cultured malignant cells. The protein encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to GM3 to produce gangliosides GD3 and GT3. The encoded protein may be found in the Golgi apparatus and is a member of glycosyltransferase family 29. [provided by RefSeq, Jul 2008]

Uniprot Description

ST8SIA1: Involved in the production of gangliosides GD3 and GT3 from GM3; gangliosides are a subfamily of complex glycosphinglolipds that contain one or more residues of sialic acid. Belongs to the glycosyltransferase 29 family.

Protein type: Transferase; Glycan Metabolism - glycosphingolipid biosynthesis - ganglio series; Glycan Metabolism - glycosphingolipid biosynthesis - lacto and neolacto series; Glycan Metabolism - glycosphingolipid biosynthesis - globo series; Membrane protein, integral; EC 2.4.99.8

Chromosomal Location of Human Ortholog: 12p12.1-p11.2

Cellular Component: Golgi membrane; integral to membrane; integral to Golgi membrane

Molecular Function: alpha-N-acetylneuraminate alpha-2,8-sialyltransferase activity; sialyltransferase activity

Biological Process: cellular protein metabolic process; glycosphingolipid biosynthetic process; dolichol-linked oligosaccharide biosynthetic process; positive regulation of cell proliferation; carbohydrate metabolic process; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification

Research Articles on ST8SIA1

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Product Notes

The ST8SIA1 st8sia1 (Catalog #AAA8121922) is a Recombinant Protein produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Thr49-Ser3 56. It is sometimes possible for the material contained within the vial of "ST8SIA1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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