Western Blot (WB)
(Figure 1. Western blot analysis of HOXC8 using anti-HOXC8 antibody (MBS1753735).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human COLO-320 whole cell lysatesLane 2: human U20S whole cell lysatesLane 3: human A549 whole cell lysatesLane 4: rat brain tissue lysatesLane 5: mouse brain tissue lysatesLane 6: mouse ovary tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-HOXC8 antigen affinity purified polyclonal antibody (Catalog # MBS1753735) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for HOXC8 at approximately 35KD. The expected band size for HOXC8 is at 35KD. )
Rabbit HOXC8 Polyclonal Antibody | anti-HOXC8 antibody
Anti-HOXC8 Antibody
FC/FACS/FCM: 1-3ug/1x106 cells|Human|
Direct ELISA: 0.1-0.5ug/ml|Human|
Western Blot (WB)
(Figure 1. Western blot analysis of HOXC8 using anti-HOXC8 antibody (MBS1753735).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human COLO-320 whole cell lysatesLane 2: human U20S whole cell lysatesLane 3: human A549 whole cell lysatesLane 4: rat brain tissue lysatesLane 5: mouse brain tissue lysatesLane 6: mouse ovary tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-HOXC8 antigen affinity purified polyclonal antibody (Catalog # MBS1753735) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for HOXC8 at approximately 35KD. The expected band size for HOXC8 is at 35KD. )
Western Blot (WB)
(Figure 1. Western blot analysis of HOXC8 using anti-HOXC8 antibody (MBS1753735).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human COLO-320 whole cell lysatesLane 2: human U20S whole cell lysatesLane 3: human A549 whole cell lysatesLane 4: rat brain tissue lysatesLane 5: mouse brain tissue lysatesLane 6: mouse ovary tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-HOXC8 antigen affinity purified polyclonal antibody (Catalog # MBS1753735) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for HOXC8 at approximately 35KD. The expected band size for HOXC8 is at 35KD. )
Flow Cytometry (FC/FACS)
(Figure 2. Flow Cytometry analysis of U251 cells using anti-HOXC8 antibody (MBS1753735).Overlay histogram showing U251 cells stained with MBS1753735 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HOXC8 Antibody (MBS1753735, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 2. Flow Cytometry analysis of U251 cells using anti-HOXC8 antibody (MBS1753735).Overlay histogram showing U251 cells stained with MBS1753735 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HOXC8 Antibody (MBS1753735, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
2. Cannizzaro, L. A., Croce, C. M., Griffin, C. A., Simeone, A., Boncinelli, E., Huebner, K. Human homeo box-containing genes located at chromosome regions 2q31-2q37 and 12q12-12q13. Am. J. Hum. Genet. 41: 1-15, 1987.
3. Masuda, R., Yuhki, N., O'Brien, S. J. Molecular cloning, chromosomal assignment, and nucleotide sequence of the feline homeobox HOX3A. Genomics 11: 1007-1013, 1991.
NCBI and Uniprot Product Information
NCBI Description
This gene belongs to the homeobox family of genes. The homeobox genes encode a highly conserved family of transcription factors that play an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters, HOXA, HOXB, HOXC and HOXD, which are located on different chromosomes and consist of 9 to 11 genes arranged in tandem. This gene is one of several homeobox HOXC genes located in a cluster on chromosome 12. The product of this gene may play a role in the regulation of cartilage differentiation. It could also be involved in chondrodysplasias or other cartilage disorders. [provided by RefSeq, Jul 2008]
Uniprot Description
HOXC8: Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Belongs to the Antp homeobox family.
Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 12q13.3
Cellular Component: microtubule cytoskeleton; nucleoplasm
Molecular Function: sequence-specific DNA binding; transcription factor activity
Biological Process: neuron differentiation; anterior/posterior pattern formation; transcription, DNA-dependent; skeletal morphogenesis; negative regulation of transcription from RNA polymerase II promoter
Research Articles on HOXC8
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Product Notes
The HOXC8 hoxc8 (Catalog #AAA1753735) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-HOXC8 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HOXC8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Flow Cytometry (FC/FACS/FCM), Direct ELISA (EIA). WB: 0.25-0.5ug/ml|Human, Mouse, Rat| FC/FACS/FCM: 1-3ug/1x106 cells|Human| Direct ELISA: 0.1-0.5ug/ml|Human|. Researchers should empirically determine the suitability of the HOXC8 hoxc8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HOXC8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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