Immunofluorescence (IF)
(Fluorescent image of Hela cell stained with HOXC10 Antibody (C-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HOXC10 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C).HOXC10 immunoreactivity is localized to Nucleus significantly. )
Rabbit anti-Human HOXC10 Polyclonal Antibody | anti-HOXC10 antibody
HOXC10, CT (HOXC10, HOX3I, Homeobox protein Hox-C10, Homeobox protein Hox-3I) (APC)
Gene Names
HOXC10; HOX3I
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
HOXC10; Polyclonal Antibody; CT (HOXC10; HOX3I; Homeobox protein Hox-C10; Homeobox protein Hox-3I) (APC); Homeobox protein Hox-3I; anti-HOXC10 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-HOXC10 antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
HOXC10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 224-250 amino acids from the C-terminal region of human HOXC10.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Immunofluorescence (IF)
(Fluorescent image of Hela cell stained with HOXC10 Antibody (C-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HOXC10 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C).HOXC10 immunoreactivity is localized to Nucleus significantly. )
Immunofluorescence (IF)
(Fluorescent image of Hela cell stained with HOXC10 Antibody (C-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HOXC10 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C).HOXC10 immunoreactivity is localized to Nucleus significantly. )
Western Blot (WB)
(HOXC10 Antibody (C-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the HOXC10 antibody detected the HOXC10 protein (arrow).)
Western Blot (WB)
(HOXC10 Antibody (C-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the HOXC10 antibody detected the HOXC10 protein (arrow).)
Related Product Information for anti-HOXC10 antibody
This gene belongs to the homeobox family of genes. The homeobox genes encode a highly conserved family of transcription factors that play an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters, HOXA, HOXB, HOXC and HOXD, which are located on different chromosomes and consist of 9 to 11 genes arranged in tandem. This gene is one of several homeobox HOXC genes located in a cluster on chromosome 12. The protein level is controlled during cell differentiation and proliferation, which may indicate this protein has a role in origin activation.
Product Categories/Family for anti-HOXC10 antibody
References
Hwang, J.H., et al. Cloning Stem Cells 11(2):269-279(2009). Zhai, Y., et al. Cancer Res. 67(21):10163-10172(2007). Gabellini, D., et al. EMBO J. 22(14):3715-3724(2003). Kosaki, K., et al. Teratology 65(2):50-62(2002). Sandrock, B., et al. J. Biol. Chem. 276(38):35328-35333(2001).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
38,073 Da
NCBI Official Full Name
homeobox protein Hox-C10
NCBI Official Synonym Full Names
homeobox C10
NCBI Official Symbol
HOXC10
NCBI Official Synonym Symbols
HOX3I
NCBI Protein Information
homeobox protein Hox-C10
UniProt Protein Name
Homeobox protein Hox-C10
Protein Family
UniProt Gene Name
HOXC10
UniProt Synonym Gene Names
HOX3I
UniProt Entry Name
HXC10_HUMAN
NCBI Description
This gene belongs to the homeobox family of genes. The homeobox genes encode a highly conserved family of transcription factors that play an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters, HOXA, HOXB, HOXC and HOXD, which are located on different chromosomes and consist of 9 to 11 genes arranged in tandem. This gene is one of several homeobox HOXC genes located in a cluster on chromosome 12. The protein level is controlled during cell differentiation and proliferation, which may indicate this protein has a role in origin activation. [provided by RefSeq, Jul 2008]
Research Articles on HOXC10
Similar Products
Product Notes
The HOXC10 hoxc10 (Catalog #AAA6307958) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HOXC10, CT (HOXC10, HOX3I, Homeobox protein Hox-C10, Homeobox protein Hox-3I) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HOXC10 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the HOXC10 hoxc10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HOXC10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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