Rabbit anti-Human, Mouse Hox A9 Polyclonal Antibody | anti-HOXA9 antibody

Hox A9

Cat. Num. AAA610571

• Gene Names: HOXA9; HOX1; ABD-B; HOX1G; HOX1.7; MGC1934
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Hox A9; Polyclonal Antibody; Anti -Hox A9; anti-HOXA9 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse Hox A9 at ~36kD.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%.

• Applicable Applications for anti-HOXA9 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Suitable for use in Western Blot, Immunofluorescence and EMSA Complex Ablation.; Dilution: Western Blot: 0.5-2ug/ml. Detects Hox A9 in C2C12 cell lysates treated with PV. C2C12 cell lysates treated with PV were resolved by electrophoresis, transferred to PVDF and probed with H7023. Proteins were visualized using a goat anti-rabbit IgG labeled with HRP and a chemiluminescence detection system.; Immunofluorescence: Detects Hox A9 in fixed cells.; EMSA Complex Ablation: Disrupts DNA-protein complexes containing Hox A9 in EMSA.
• Immunogen: GST fusion protein corresponding to residues 194-272 of mouse Hox A9. The immunizing sequence is identical in human, chicken and guinea pig.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(WB: Lysate from C2C12 cells treated with PV was probed with MBS610571 (1ug/ml).)

Related Product Information for anti-HOXA9 antibody

Homeobox protein Hox A9 is a protein that in humans is encoded by the HOXA9 gene. In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. This gene is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation. This gene is highly similar to the abdominal-B (Abd-B) gene of Drosophila. A specific translocation event which causes a fusion between this gene and the NUP98 gene has been associated with myeloid leukemogenesis.

Product Categories/Family for anti-HOXA9 antibody

Antibodies; Abs to Homeobox (HOX)

NCBI and Uniprot Product Information

• NCBI GI #: 23097236
• NCBI GeneID: 3205
• NCBI Accession #: NP_689952.1
• NCBI GenBank Nucleotide #: NM_152739.3
• UniProt Accession #: P31269; O43369; O43429; Q99820; O75805; O75806
• Molecular Weight: 30,172 Da
• NCBI Official Full Name: homeobox protein Hox-A9
• NCBI Official Synonym Full Names: homeobox A9
• NCBI Official Symbol: HOXA9
• NCBI Official Synonym Symbols: HOX1; ABD-B; HOX1G; HOX1.7; MGC1934
• NCBI Protein Information: homeobox protein Hox-A9; homeo box A9; OTTHUMP00000217260; homeobox protein HOXA9; homeobox protein Hox-1G; homeodomain protein HOXA9
• UniProt Protein Name: Homeobox protein Hox-A9
• Protein Family: Homeobox protein
• UniProt Gene Name: HOXA9
• UniProt Synonym Gene Names: HOX1G
• UniProt Entry Name: HXA9_HUMAN

NCBI Description

In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. This gene is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation. This gene is highly similar to the abdominal-B (Abd-B) gene of Drosophila. A specific translocation event which causes a fusion between this gene and the NUP98 gene has been associated with myeloid leukemogenesis. Read-through transcription exists between this gene and the upstream homeobox A10 (HOXA10) gene.[provided by RefSeq]

Uniprot Description

HOXA9: Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Required for induction of E-selectin and VCAM-1, on the endothelial cells surface at sites of inflammation. A chromosomal aberration involving HOXA9 is found in a form of acute myeloid leukemia. Translocation t(7;11)(p15;p15) with NUP98. A chromosomal aberration involving HOXA9 may contribute to disease progression in chronic myeloid leukemia. Translocation t(7;17)(p15;q23) with MSI2. Belongs to the Abd-B homeobox family.

Protein type: DNA-binding; Oncoprotein

Chromosomal Location of Human Ortholog: 7p15.2

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleus

Molecular Function: protein binding; enzyme binding; sequence-specific DNA binding

Biological Process: embryonic forelimb morphogenesis; negative regulation of myeloid cell differentiation; transcription, DNA-dependent; embryonic skeletal development; multicellular organismal development; male gonad development; uterus development; endothelial cell activation; anterior/posterior pattern formation; mammary gland development; regulation of transcription, DNA-dependent; single fertilization; spermatogenesis; proximal/distal pattern formation