Rabbit anti-Human HOXA3 Polyclonal Antibody | anti-HOXA3 antibody

HOXA3, CT (HOXA3, HOX1E, Homeobox protein Hox-A3, Homeobox protein Hox-1E) (Azide free) (HRP)

Cat. Num. AAA6307719

• Gene Names: HOXA3; HOX1; HOX1E
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: HOXA3; Polyclonal Antibody; CT (HOXA3; HOX1E; Homeobox protein Hox-A3; Homeobox protein Hox-1E) (Azide free) (HRP); Homeobox protein Hox-1E; anti-HOXA3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-HOXA3 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: HOXA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 414~443 amino acids from the C-terminal region of human HOXA3.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of HOXA3 Antibody (C-term) in HL-60 cell line lysates (35ug/lane). HOXA3 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(HOXA3 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HOXA3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(HOXA3 Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-HOXA3 antibody

In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. This gene is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation.

Product Categories/Family for anti-HOXA3 antibody

Antibodies; Transcription Factors; Zinc; Ring; Homeobox

References

Yerges, L.M., et al. J. Bone Miner. Res. 24(12):2039-2049(2009). Chung, N., et al. Mol. Biol. Rep. 36(2):227-235(2009). Wissmuller, S., et al. Nucleic Acids Res. 34(6):1735-1744(2006). Manzanares, M., et al. EMBO J. 21(3):365-376(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 6016293
• NCBI GeneID: 3200
• UniProt Accession #: O43365; A4D181
• Molecular Weight: 46,369 Da
• NCBI Official Full Name: Homeobox protein Hox-A3
• NCBI Official Synonym Full Names: homeobox A3
• NCBI Official Symbol: HOXA3
• NCBI Official Synonym Symbols: HOX1; HOX1E
• NCBI Protein Information: homeobox protein Hox-A3; homeo box 1E; homeo box A3; Hox-1.5-like protein; homeobox protein Hox-1E
• UniProt Protein Name: Homeobox protein Hox-A3
• Protein Family: Homeobox protein
• UniProt Gene Name: HOXA3
• UniProt Synonym Gene Names: HOX1E
• UniProt Entry Name: HXA3_HUMAN

NCBI Description

In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. This gene is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

HOXA3: Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Belongs to the Antp homeobox family.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 7p15.2

Cellular Component: nucleoplasm

Molecular Function: sequence-specific DNA binding; transcription factor activity

Biological Process: anterior/posterior pattern formation; specification of organ position; organ formation; regulation of transcription, DNA-dependent; transcription, DNA-dependent; thymus development; cartilage development; positive regulation of cell proliferation; glossopharyngeal nerve morphogenesis; thyroid gland development; blood vessel remodeling; angiogenesis; parathyroid gland development; embryonic skeletal morphogenesis