Western Blot
(Western blot analysis using MBS520822 (Affinity purified rabbit anti-HRP) at a 1/8000 dilution on 1) 0.2 ug and 2) 0.1 ug of purified HRP.)
Rabbit Horseradish Peroxidase (HRP) Polyclonal Antibody | anti-HRP antibody
Anti-Horseradish Peroxidase (HRP), Purified, (Polyclonal), (Rabbit IgG)
Applications
ELISA, Western Blot
Purity
Purified from serum via Affinity Chromatography.
Synonyms
Horseradish Peroxidase (HRP); Polyclonal Antibody; Anti-Horseradish Peroxidase (HRP); Purified; (Polyclonal); (Rabbit IgG); anti-HRP antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Specificity
This antibody is specific for horseradish peroxidase.
Purity/Purification
Purified from serum via Affinity Chromatography.
Form/Format
Purified IgG buffered in PBS and 0.02% NaN3
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
353
Applicable Applications for anti-HRP antibody
ELISA (EIA), Western Blot (WB)
Application Notes
ELISA: (1:4K - 1:8K dilution)
Western Blot: (1:4K - 1:8K dilution).
This antibody has not been tested in other applications.
Appropriate control samples should always be included in any labeling studies.
For optimal results in various applications, it is recommended that each investigator determine dilutions appropriate for individual use.
Western Blot: (1:4K - 1:8K dilution).
This antibody has not been tested in other applications.
Appropriate control samples should always be included in any labeling studies.
For optimal results in various applications, it is recommended that each investigator determine dilutions appropriate for individual use.
Immunogen
Purified horseradish peroxidase.
Notes
For maximum recovery of contents, spin down tube before use.
Preparation and Storage
For all formats, store at + 4 degree C. For long term storage, aliquot and freeze unused portion at -20 degree C in volumes appropriate for single usage. Avoid freeze/thaw cycles.
Western Blot
(Western blot analysis using MBS520822 (Affinity purified rabbit anti-HRP) at a 1/8000 dilution on 1) 0.2 ug and 2) 0.1 ug of purified HRP.)
Western Blot
(Western blot analysis using MBS520822 (Affinity purified rabbit anti-HRP) at a 1/8000 dilution on 1) 0.2 ug and 2) 0.1 ug of purified HRP.)
Related Product Information for anti-HRP antibody
Horseradish peroxidase (HRP) is a metalloenzyme found in the root of horseradish that is frequently used as an indicator in biochemical assays. HRP is often conjugated to antibodies and is used primarily for its ability to amplify a signal and increase detectability of a target molecule. It acts on a number of chromogenic and chemiluminescent substrates to produce different colors and light respectively.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
horseradish peroxidase C1a
Similar Products
Product Notes
The HRP (Catalog #AAA520822) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Horseradish Peroxidase (HRP) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). ELISA: (1:4K - 1:8K dilution) Western Blot: (1:4K - 1:8K dilution). This antibody has not been tested in other applications. Appropriate control samples should always be included in any labeling studies. For optimal results in various applications, it is recommended that each investigator determine dilutions appropriate for individual use. Researchers should empirically determine the suitability of the HRP for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Horseradish Peroxidase (HRP), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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