Chicken Hormone Sensitive Lipase Polyclonal Antibody | anti-LIPE antibody

Hormone Sensitive Lipase (HSL)

Cat. Num. AAA616664

• Gene Names: LIPE; HSL; LHS
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Purified; Purified immunoglobulin
• Synonyms: Hormone Sensitive Lipase; Polyclonal Antibody; Hormone Sensitive Lipase (HSL); Anti -Hormone Sensitive Lipase (HSL); anti-LIPE antibody

• Host: Chicken
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgY
• Specificity: Binds to a band at about 83kD (HSL) in various fat cell lysates from mouse and rat, and an additional band at about 40kD (unknown). Species Crossreactivity: human, rat and mouse.
• Purity/Purification: Purified; Purified immunoglobulin
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide.

• Applicable Applications for anti-LIPE antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot and ELISA.; Dilution: ELISA: 1:1000-1:5000; Western Blot: 1:1000-1:5000
• Immunogen: HSL mixed peptides (NSQEEAEAKDEV, SGRKPQKTTSPT and FGEHYKRNETGL)
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-LIPE antibody

Lipase, hormone-sensitive also known as LIPE is an enzyme that, in humans, is encoded by the LIPE gene. LIPE is an intracellular neutral lipase that is capable of hydrolyzing a variety of esters.[2] The enzyme has a long and a short form. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids.

LIPE functions to hydrolyze the first fatty acid from a triacylglycerol molecule, freeing a fatty acid and diglyceride. It is also known as triglyceride lipase, while the enzyme that cleaves the second fatty acid in the triglyceride is known as diglyceride lipase, and the third enzyme that cleaves the final fatty acid is called monoglyceride lipase. Only the initial enzyme is affected by hormones, hence its hormone-sensitive lipase name. The diglyceride and monoglyceride enzymes are tens to hundreds of times faster, hence HSL is the rate-limiting step in cleaving fatty acids from the triglyceride molecule.
LIPE is activated when the body needs to mobilize energy stores, and so responds positively to catecholamines, ACTH, and negatively to insulin. Previously, glucagon was thought to activate LIPE, however the removal of insulin's inhibitory effects ("cutting the brakes") is the source of activation. The lipolytic effect of glucagon in adipose tissue is minimal in humans. LIPE may be activated by two mechanisms. In the first, phosphorylated perilipin A causes it to move to the surface of the lipid droplet, where it may begin hydrolyzing the lipid droplet. Also, it may be activated by a cAMP-dependent protein kinase (PKA). This pathway is significantly less effective than the first, which is necessary to lipid mobilization in response to cyclic AMP, which itself is provided by beta adrenergic stimulation of the glucagon receptor.

Product Categories/Family for anti-LIPE antibody

Antibodies; Abs to Enzymes; Lipase

NCBI and Uniprot Product Information

• NCBI GI #: 21328446
• NCBI GeneID: 3991
• NCBI Accession #: NP_005348.2
• NCBI GenBank Nucleotide #: NM_005357.2
• UniProt Accession #: Q05469; Q3LRT2; Q6NSL7
• Molecular Weight: 116,598 Da
• NCBI Official Full Name: hormone-sensitive lipase
• NCBI Official Synonym Full Names: lipase, hormone-sensitive
• NCBI Official Symbol: LIPE
• NCBI Official Synonym Symbols: HSL; LHS
• NCBI Protein Information: hormone-sensitive lipase; hormone-sensitive lipase testicular isoform
• UniProt Protein Name: Hormone-sensitive lipase
• Protein Family: Hormone-sensitive lipase
• UniProt Gene Name: LIPE
• UniProt Entry Name: LIPS_HUMAN

NCBI Description

The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids. [provided by RefSeq]

Uniprot Description

HSL: hormone sensitive lipase is a lipolytic enzyme of the 'GDXG' family. Plays a rate limiting step in triglyceride lipolysis. In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production. Rapidly activated by cAMP-dependent phosphorylation under the influence of catecholamines. Dephosphorylation and inactivation are controlled by insulin.

Protein type: Lipase; EC 3.1.1.79

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: lipid particle; caveola; cytosol

Molecular Function: triacylglycerol lipase activity; hormone-sensitive lipase activity; protein binding; protein kinase binding

Biological Process: cholesterol metabolic process; triacylglycerol catabolic process; diacylglycerol catabolic process; protein amino acid phosphorylation; lipid catabolic process; long-chain fatty acid catabolic process

Disease: Abdominal Obesity-metabolic Syndrome 4