Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Transcription factor Maf (MAF) Standard Curve.)
Mouse Transcription Factor Maf (MAF) ELISA Kit | MAF elisa kit
Mouse Transcription Factor Maf (MAF) ELISA Kit
Gene Names
Maf; c-maf; AW047063; 2810401A20Rik; A230108G15Rik
Reactivity
Mouse
Synonyms
Transcription Factor Maf (MAF); Mouse Transcription Factor Maf (MAF) ELISA Kit; MAF; MGC71685; c-MAF; Avian musculoaponeurotic fibrosarcoma (MAF) protooncogene; T lymphocyte c-maf long form; c-maf proto-oncogene; v-maf musculoaponeurotic fibrosarcoma oncogene homolog; MAF elisa kit
Reactivity
Mouse
Specificity
Mouse Transcription factor Maf (MAF) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MAF. No significant cross-reactivity or interference between Mouse MAF and analogues was observed.
Sequence Length
370
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Transcription factor Maf (MAF) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Transcription factor Maf (MAF) Standard Curve.)
Related Product Information for MAF elisa kit
Description: This Mouse Transcription factor Maf (MAF) ELISA Kit employs a two-site sandwich ELISA to quantitate MAF in samples. An antibody specific for MAF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MAF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAF is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAF bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The protein encoded by MAF is a DNA-binding, leucine zipper-containing transcription factor that acts as a homodimer or as a heterodimer. Depending on the binding site and binding partner, the encoded protein can be a transcriptional activator or repressor. This protein plays a role in the regulation of several cellular processes, including embryonic lens fiber cell development, increased T-cell susceptibility to apoptosis, and chondrocyte terminal differentiation. Defects in MAF are a cause of juvenile-onset pulverulent cataract as well as congenital cerulean cataract 4 (CCA4). Two transcript variants encoding different isoforms have been found for MAF.
Background: The protein encoded by MAF is a DNA-binding, leucine zipper-containing transcription factor that acts as a homodimer or as a heterodimer. Depending on the binding site and binding partner, the encoded protein can be a transcriptional activator or repressor. This protein plays a role in the regulation of several cellular processes, including embryonic lens fiber cell development, increased T-cell susceptibility to apoptosis, and chondrocyte terminal differentiation. Defects in MAF are a cause of juvenile-onset pulverulent cataract as well as congenital cerulean cataract 4 (CCA4). Two transcript variants encoding different isoforms have been found for MAF.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
transcription factor Maf
NCBI Official Synonym Full Names
avian musculoaponeurotic fibrosarcoma oncogene homolog
NCBI Official Symbol
Maf
NCBI Official Synonym Symbols
c-maf; AW047063; 2810401A20Rik; A230108G15Rik
NCBI Protein Information
transcription factor Maf
UniProt Protein Name
Transcription factor Maf
Protein Family
UniProt Gene Name
Maf
UniProt Synonym Gene Names
Maf2
UniProt Entry Name
MAF_MOUSE
Research Articles on MAF
1. this study shows that c-Maf is essential for the commitment of IL-17-producing gammadelta T cells
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Product Notes
The Mouse MAF maf (Catalog #AAA9717748) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717748 ELISA Kit recognizes Mouse MAF. It is sometimes possible for the material contained within the vial of "Transcription Factor Maf (MAF), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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