HOMER3 recombinant protein

HOMER3, 1-361aa, Human, His tag, E Coli

Cat. Num. AAA204635

• Gene Names: HOMER3; VESL3; HOMER-3
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: HOMER3; 1-361aa; Human; His tag; E Coli; Homer protein homolog 3; HOMER-3; VESL3; HOMER3 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 30% glycerol, 0.15M NaCl, 1mM DTT
• Concentration: 0.25 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMMSTAR EQPIFSTRAH VFQIDPATKR NWIPAGKHAL TVSYFYDATR NVYRIISIGG AKAIINSTVT PNMTFTKTSQ KFGQWADSRA NTVYGLGFAS EQHLTQFAEK FQEVKEAARL AREKSQDGGE LTSPALGLAS HQVPPSPLVS ANGPGEEKLF RSQSADAPGP TERERLKKML SEGSVGEVQW EAEFFALQDS NNKLAGALRE ANAAAAQWRQ QLEAQRAEAE RLRQRVAELE AQAASEVTPT GEKEGLGQGQ SLEQLEALVQ TKDQEIQTLK SQTGGPREAL EAAEREETQQ KVQDLETRNA ELEHQLRAME RSLEEARAER ERARAEVGRA AQLLDVSLFE LSELREGLAR LAEAAP
• Sequence Length: 361

• Applicable Applications for HOMER3 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for HOMER3 recombinant protein

HOMER3, also known as homer protein homolog 3, is a member of the HOMER family of postsynaptic density scaffolding proteins that share a similar domain structure consisting of an N-terminal Enabled/vasodilator-stimulated phosphoprotein homology 1 domain which mediates protein-protein interactions, and a carboxy-terminal coiled-coil domain and two leucine zipper motifs that are involved in self-oligomerization. Recombinant human HOMER3 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography.

Product Categories/Family for HOMER3 recombinant protein

Neuroscience

References

Xiao B., et al. (1998) Neuron. 21:707-716; Mayya V., et al. (2009) Sci. Signal. 2:RA46-RA46

NCBI and Uniprot Product Information

• NCBI GI #: 224809414
• NCBI GeneID: 9454
• NCBI Accession #: NP_001139194
• NCBI GenBank Nucleotide #: NM_001145722.1
• UniProt Accession #: Q9NSC5; O14580; O95350; Q9NSB9; Q9NSC0; Q9NSC1; B2RA10; E9PCW9
• Molecular Weight: 42.5 kDa (386aa), confirmed by MALDI-TOF
• NCBI Official Full Name: homer protein homolog 3 isoform 1
• NCBI Official Synonym Full Names: homer scaffolding protein 3
• NCBI Official Symbol: HOMER3
• NCBI Official Synonym Symbols: VESL3; HOMER-3
• NCBI Protein Information: homer protein homolog 3
• UniProt Protein Name: Homer protein homolog 3
• Protein Family: Homer protein
• UniProt Gene Name: HOMER3
• UniProt Synonym Gene Names: Homer-3

NCBI Description

This gene encodes a member of the HOMER family of postsynaptic density scaffolding proteins that share a similar domain structure consisting of an N-terminal Enabled/vasodilator-stimulated phosphoprotein homology 1 domain which mediates protein-protein interactions, and a carboxy-terminal coiled-coil domain and two leucine zipper motifs that are involved in self-oligomerization. The encoded protein binds numerous other proteins including group I metabotropic glutamate receptors, inositol 1,4,5-trisphosphate receptors and amyloid precursor proteins and has been implicated in diverse biological functions such as neuronal signaling, T-cell activation and trafficking of amyloid beta peptides. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Mar 2009]

Uniprot Description

Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. Isoforms can be differently regulated and may play an important role in maintaining the plasticity at glutamatergic synapses.