Rabbit anti-Human HNRPDL Polyclonal Antibody | anti-HNRNPDL antibody

HNRPDL, ID (HNRPDL, JKTBP, Heterogeneous nuclear ribonucleoprotein D-like, AU-rich element RNA-binding factor, JKT41-binding protein, Protein laAUF1)

Cat. Num. AAA648170

• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: HNRPDL; Polyclonal Antibody; ID (HNRPDL; JKTBP; Heterogeneous nuclear ribonucleoprotein D-like; AU-rich element RNA-binding factor; JKT41-binding protein; Protein laAUF1); Anti -HNRPDL; anti-HNRNPDL antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-HNRNPDL antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500
• Immunogen: HNRPDL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 214-244 amino acids from the Central region of human HNRPDL.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(HNRPDL Antibody (Center) western blot analysis in K562,HepG2,Jurkat cell line lysates (35ug/lane).This demonstrates the HNRPDL antibody detected the HNRPDL protein (arrow).)

Related Product Information for anti-HNRNPDL antibody

HNRPDL belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein has two RRM domains that bind to RNAs.

Product Categories/Family for anti-HNRNPDL antibody

Antibodies; Abs to Nuclear Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 47115183
• UniProt Accession #: O14979; Q6SPF2; Q7KZ74; Q7KZ75; Q96IM0; Q96S43
• Molecular Weight: 46,438 Da
• NCBI Official Full Name: HNRPDL
• UniProt Protein Name: Heterogeneous nuclear ribonucleoprotein D-like
• Protein Family: Heterogeneous nuclear ribonucleoprotein
• UniProt Gene Name: HNRNPDL
• UniProt Synonym Gene Names: HNRPDL; JKTBP; hnRNP D-like; hnRNP DL
• UniProt Entry Name: HNRDL_HUMAN

Uniprot Description

Function: Acts as a transcriptional regulator. Promotes transcription repression. Promotes transcription activation in differentiated myotubes

By similarity. Binds to double- and single-stranded DNA sequences. Binds to the transcription suppressor CATR sequence of the COX5B promoter

By similarity. Binds with high affinity to RNA molecules that contain AU-rich elements (AREs) found within the 3'-UTR of many proto-oncogenes and cytokine mRNAs. Binds both to nuclear and cytoplasmic poly(A) mRNAs. Binds to poly(G) and poly(A), but not to poly(U) or poly(C) RNA homopolymers. Binds to the 5'-ACUAGC-3' RNA consensus sequence. Ref.1

Subunit structure: Interacts with ZNF148

By similarity. Interacts with TNPO1. Ref.8

Subcellular location: Nucleus. Cytoplasm. Note: Shuttles between the nucleus and the cytoplasm in a TNPO1-dependent manner. Ref.3 Ref.11

Tissue specificity: Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and leukocytes. Expressed in myeloid leukemia, gastric adenocarcinoma, cervical carcinoma, hepatoma, fibrosarcoma, colon adenocarcinoma, epidermoid carcinoma, osteosarcoma and urinary bladder carcinoma cells. Ref.1 Ref.2

Induction: Up-regulated by 12-O-tetradecanoylphorbol-13-acetate (TPA) in macrophages and retinoic acid (RA) in granulocytes (at protein level). Down-regulated by IL4/interleukin-4. Ref.2 Ref.8

Post-translational modification: Dimethylation of Arg-408 is probably of the asymmetric type.

Sequence similarities: Contains 2 RRM (RNA recognition motif) domains.

Sequence caution: The sequence BAA22860.1 differs from that shown. Reason: Erroneous initiation.