Rabbit anti-Human HNRPDL Polyclonal Antibody | anti-HNRPDL antibody

HNRPDL, ID (HNRPDL, JKTBP, Heterogeneous nuclear ribonucleoprotein D-like, AU-rich element RNA-binding factor, JKT41-binding protein, Protein laAUF1) (Azide free) (HRP)

Cat. Num. AAA6307565

• Gene Names: HNRNPDL; HNRNP; JKTBP; HNRPDL; JKTBP2; LGMD1G; laAUF1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: HNRPDL; Polyclonal Antibody; ID (HNRPDL; JKTBP; Heterogeneous nuclear ribonucleoprotein D-like; AU-rich element RNA-binding factor; JKT41-binding protein; Protein laAUF1) (Azide free) (HRP); Protein laAUF1; anti-HNRPDL antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-HNRPDL antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: HNRPDL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 214-244 amino acids from the Central region of human HNRPDL.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(HNRPDL Antibody (Center) western blot analysis in K562,HepG2,Jurkat cell line lysates (35ug/lane).This demonstrates the HNRPDL antibody detected the HNRPDL protein (arrow).)

Related Product Information for anti-HNRPDL antibody

HNRPDL belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein has two RRM domains that bind to RNAs.

Product Categories/Family for anti-HNRPDL antibody

Antibodies; Nuclear Proteins

References

Walker, L.C., et al. Breast Cancer Res. Treat. 112(2):229-236(2008). Wu, Y.Y., et al. Cell Biochem. Funct. 26(4):467-477(2008). Reboll, M.R., et al. RNA 13(8):1328-1340(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 14110407
• NCBI GeneID: 9987
• NCBI Accession #: NP_112740.1
• NCBI GenBank Nucleotide #: NM_031372.3
• UniProt Accession #: O14979; Q6SPF2; Q7KZ74; Q7KZ75; Q96IM0; Q96S43
• Molecular Weight: 27,191 Da
• NCBI Official Full Name: heterogeneous nuclear ribonucleoprotein D-like isoform a
• NCBI Official Synonym Full Names: heterogeneous nuclear ribonucleoprotein D like
• NCBI Official Symbol: HNRNPDL
• NCBI Official Synonym Symbols: HNRNP; JKTBP; HNRPDL; JKTBP2; LGMD1G; laAUF1
• NCBI Protein Information: heterogeneous nuclear ribonucleoprotein D-like
• UniProt Protein Name: Heterogeneous nuclear ribonucleoprotein D-like
• UniProt Gene Name: HNRNPDL
• UniProt Synonym Gene Names: HNRPDL; JKTBP; hnRNP D-like; hnRNP DL

NCBI Description

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two RRM domains that bind to RNAs. Three alternatively spliced transcript variants have been described for this gene. One of the variants is probably not translated because the transcript is a candidate for nonsense-mediated mRNA decay. The protein isoforms encoded by this gene are similar to its family member HNRPD. [provided by RefSeq, May 2011]

Uniprot Description

Acts as a transcriptional regulator. Promotes transcription repression. Promotes transcription activation in differentiated myotubes (). Binds to double- and single-stranded DNA sequences. Binds to the transcription suppressor CATR sequence of the COX5B promoter (). Binds with high affinity to RNA molecules that contain AU-rich elements (AREs) found within the 3'-UTR of many proto-oncogenes and cytokine mRNAs. Binds both to nuclear and cytoplasmic poly(A) mRNAs. Binds to poly(G) and poly(A), but not to poly(U) or poly(C) RNA homopolymers. Binds to the 5'-ACUAGC-3' RNA consensus sequence.