Rabbit anti-Human, Mouse UBE2W Polyclonal Antibody | anti-UBE2W antibody

UBE2W, CT (UBE2W, UBC16, Ubiquitin-conjugating enzyme E2 W, Ubiquitin carrier protein W, Ubiquitin-conjugating enzyme 16, Ubiquitin-protein ligase W) (PE)

Cat. Num. AAA6360848

• Gene Names: UBE2W; UBC16; UBC-16
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: UBE2W; Polyclonal Antibody; CT (UBE2W; UBC16; Ubiquitin-conjugating enzyme E2 W; Ubiquitin carrier protein W; Ubiquitin-conjugating enzyme 16; Ubiquitin-protein ligase W) (PE); Ubiquitin-protein ligase W; anti-UBE2W antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Sequence Length: 162

• Applicable Applications for anti-UBE2W antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: UBE2W antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-133 amino acids from the C-terminal region of human UBE2W.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(UBE2W Antibody (C-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the UBE2W antibody detected the UBE2W protein (arrow).)

Western Blot (WB)

(UBE2W Antibody (C-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the UBE2W antibody detected the UBE2W protein (arrow).)

Immunohistochemistry (IHC)

(UBE2W Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UBE2W Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-UBE2W antibody

UBE2W accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes monoubiquitination and 'Lys-11'-linked polyubiquitination.

Product Categories/Family for anti-UBE2W antibody

Antibodies; Ubiquitin Ligases

References

Markson, G., et al. Genome Res. 19(10):1905-1911(2009). van Wijk, S.J., et al. Mol. Syst. Biol. 5, 295 (2009): Yin, G., et al. Front. Biosci. 11, 1500-1507 (2006).

NCBI and Uniprot Product Information

• NCBI GI #: 47933381
• NCBI GeneID: 55284
• NCBI Accession #: NP_001001481.1; NP_060769.3
• NCBI GenBank Nucleotide #: NP_001001481.1
• UniProt Accession #: Q96B02
• NCBI Official Full Name: ubiquitin-conjugating enzyme E2 W isoform 1
• NCBI Official Synonym Full Names: ubiquitin conjugating enzyme E2 W
• NCBI Official Symbol: UBE2W
• NCBI Official Synonym Symbols: UBC16; UBC-16
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 W
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 W
• Protein Family: Ubiquitin-conjugating enzyme
• UniProt Gene Name: UBE2W
• UniProt Synonym Gene Names: UBC16; UBC-16
• UniProt Entry Name: UBE2W_HUMAN

NCBI Description

This gene encodes a nuclear-localized ubiquitin-conjugating enzyme (E2) that, along with ubiquitin-activating (E1) and ligating (E3) enzymes, coordinates the addition of a ubiquitin moiety to existing proteins. The encoded protein promotes the ubiquitination of Fanconi anemia complementation group proteins and may be important in the repair of DNA damage. There is a pseudogene for this gene on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

Uniprot Description

UBE2W: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in degradation of misfolded chaperone substrates by mediating monoubiquitination of STUB1/CHIP, leading to recruitment of ATXN3 to monoubiquitinated STUB1/CHIP, and restriction of the length of ubiquitin chain attached to STUB1/CHIP substrates by ATXN3. After UV irradiation, but not after mitomycin-C (MMC) treatment, acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Belongs to the ubiquitin-conjugating enzyme family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; EC 6.3.2.19; Enzyme, misc.; Ubiquitin ligase; Ligase

Chromosomal Location of Human Ortholog: 8q21.11

Cellular Component: cytoplasm; nucleus

Molecular Function: small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ATP binding; ligase activity

Biological Process: protein monoubiquitination; proteasomal ubiquitin-dependent protein catabolic process; misfolded or incompletely synthesized protein catabolic process; DNA repair