Rabbit HNRNPF Polyclonal Antibody | anti-HNRNPF antibody

HNRNPF Antibody

Cat. Num. AAA9408266

• Gene Names: HNRNPF; HNRPF; mcs94-1; OK/SW-cl.23
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: HNRNPF; Polyclonal Antibody; HNRNPF Antibody; HNRPF; mcs94-1; OK/SW-cl.23; anti-HNRNPF antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total HNRNPF protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 415

• Applicable Applications for anti-HNRNPF antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunohistochemistry: 1:50 - 1:200; Immunofluorescence: 1:50 - 1:200
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human HNRNPF.
• Target Name: HNRNPF
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using HNRNPF antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of U20S cell using HNRNPF antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-HNRNPF antibody

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Product Categories/Family for anti-HNRNPF antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 148470404
• NCBI GeneID: 3185
• NCBI Accession #: NP_001091674.1
• NCBI GenBank Nucleotide #: NM_001098204.1
• UniProt Accession #: P52597; Q5T0N2; Q96AU2; B3KM84
• Molecular Weight: 45,672 Da
• NCBI Official Full Name: heterogeneous nuclear ribonucleoprotein F
• NCBI Official Synonym Full Names: heterogeneous nuclear ribonucleoprotein F
• NCBI Official Symbol: HNRNPF
• NCBI Official Synonym Symbols: HNRPF; mcs94-1; OK/SW-cl.23
• NCBI Protein Information: heterogeneous nuclear ribonucleoprotein F
• UniProt Protein Name: Heterogeneous nuclear ribonucleoprotein F
• Protein Family: Heterogeneous nuclear ribonucleoprotein
• UniProt Gene Name: HNRNPF
• UniProt Synonym Gene Names: HNRPF; hnRNP F
• UniProt Entry Name: HNRPF_HUMAN

NCBI Description

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

hnRNP F: Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Plays a role in the regulation of alternative splicing events. Binds G-rich sequences in pre-mRNAs and keeps target RNA in an unfolded state.

Protein type: RNA splicing; Spliceosome; RNA-binding

Chromosomal Location of Human Ortholog: 10q11.21

Cellular Component: nucleoplasm; membrane; nucleus

Molecular Function: protein binding; single-stranded RNA binding; RNA binding; nucleotide binding

Biological Process: nuclear mRNA splicing, via spliceosome; RNA processing; regulation of RNA splicing; RNA splicing; gene expression