Mouse Osteocalcin, aa4-9 Monoclonal Antibody | anti-BGLAP antibody

Osteocalcin, aa4-9 (Bone-Gla-Protein, BGP)

Cat. Num. AAA6012908

• Gene Names: BGLAP; OC; BGP; OCN
• Reactivity: Bovine, Canine, Chicken, Goat, Human, Porcine, Rabbit, Sheep
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by affinity chromatography
• Synonyms: Osteocalcin; aa4-9; Monoclonal Antibody; aa4-9 (Bone-Gla-Protein; BGP); Anti -Osteocalcin; anti-BGLAP antibody

• Host: Mouse
• Reactivity: Bovine, Canine, Chicken, Goat, Human, Porcine, Rabbit, Sheep
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 6D627
• Specificity: Recognizes aa4-9 of bovine osteocalcin. Species Crossreactivity: human, goat, sheep, canine, porcine, rabbit and chicken. Does not crossreact with mouse or rat osteocalcin.
• Purity/Purification: Affinity Purified; Purified by affinity chromatography
• Form/Format: Supplied as a lyophilized powder from PBS, pH 7.4, 1% BSA. Reconstitute with 50ul sterile ddH20. Dilute further with PBS, pH 7.4, 1% BSA, 0.1% sodium azide.

• Applicable Applications for anti-BGLAP antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Immunohistochemistry and Western Blot.; Dilution: Immunohistochemistry(Frozen/paraffin): 10ug/ml
• Immunogen: Bovine osteocalcin
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

(Figure: Recognition sites of MAbs to osteocalcin assayed by peptide fragments using MBS6012908.)

Related Product Information for anti-BGLAP antibody

Osteocalcin, also known as Bone-Gla-Protein (BGP), is a single chain (Mr=5800) vitamin K dependent protein produced by osteoblasts. It is found in high concentrations in bone. Post-translational modification by a vitamin K dependent carboxylase produces three g-carboxyglutamic acid residues at positions 17, 21 and 24. The mature protein contains 49 amino acids and a single intrachain disulfide bond joining Cys23 to Cys29. The secondary structure is highly calcium dependent and contains 14% alpha-helix, 20% beta-sheet and 67% random form in the presence of calcium, and 1% alpha-helix, 20% beta-sheet and 79% random form in the absence of calcium. BGP binds to phospholipid vesicles in the presence of calcium ions (Kd=6 x 10e-6 M). BGP also binds hydroxyapatite and is an efficient inhibitor of hydroxyapatite seeded crystal growth, suggesting a regulatory role in bone mineralization. Although the fundamental role of BGP in vivo remains elusive, in vitro experimentation suggests roles in both bone resorption and bone formation. BGP has been shown to function as a chemo-attractant in an in vitro macrophage mediated bone resorption system. The serum concentration of BGP has been linked to bone formation rates, and BGP levels are currently used in research assessment of osteoporosis and other metabolic bone disease states.

Product Categories/Family for anti-BGLAP antibody

Antibodies; Abs to Osteocalcin

NCBI and Uniprot Product Information

• NCBI GI #: 40316933
• NCBI GeneID: 632
• NCBI Accession #: NP_954642.1
• NCBI GenBank Nucleotide #: NM_199173.4
• UniProt Accession #: P02818; Q5TCK6
• Molecular Weight: 10,963 Da
• NCBI Official Full Name: osteocalcin preproprotein
• NCBI Official Synonym Full Names: bone gamma-carboxyglutamate (gla) protein
• NCBI Official Symbol: BGLAP
• NCBI Official Synonym Symbols: OC; BGP; OCN
• NCBI Protein Information: osteocalcin; bone Gla protein; gamma-carboxyglutamic acid-containing protein; bone gamma-carboxyglutamate (gla) protein (osteocalcin)
• UniProt Protein Name: Osteocalcin
• Protein Family: Osteocalcin
• UniProt Gene Name: BGLAP
• UniProt Synonym Gene Names: BGP
• UniProt Entry Name: OSTCN_HUMAN

NCBI Description

This gene encodes a highly abundant bone protein secreted by osteoblasts that regulates bone remodeling and energy metabolism. The encoded protein contains a Gla (gamma carboxyglutamate) domain, which functions in binding to calcium and hydroxyapatite, the mineral component of bone. Serum osteocalcin levels may be negatively correlated with metabolic syndrome. Read-through transcription exists between this gene and the neighboring upstream gene, PMF1 (polyamine-modulated factor 1), but the encoded protein only shows sequence identity with the upstream gene product. [provided by RefSeq, Jun 2015]

Uniprot Description

osteocalcin: Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. Belongs to the osteocalcin/matrix Gla protein family.

Protein type: Secreted; Cell development/differentiation; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q22

Cellular Component: Golgi apparatus; extracellular space; rough endoplasmic reticulum; dendrite; cytoplasm; perikaryon

Molecular Function: structural constituent of bone; hydroxyapatite binding; calcium ion binding; structural molecule activity

Biological Process: response to drug; response to gravity; regulation of bone resorption; response to glucocorticoid stimulus; cell aging; response to testosterone stimulus; bone mineralization; osteoblast development; osteoblast differentiation; odontogenesis; regulation of osteoclast differentiation; response to ethanol; response to vitamin D; response to vitamin K; response to zinc ion; response to estrogen stimulus; response to mechanical stimulus; response to hydroxyisoflavone; response to activity; cell adhesion; skeletal development; regulation of bone mineralization