General Progesterone (PG) ELISA Kit | PG elisa kit

General Progesterone (PG) ELISA Kit

Cat. Num. AAA2700150

• Gene Names: PGR; PR; NR3C3
• Reactivity: General
• Synonyms: Progesterone (PG); General Progesterone (PG) ELISA Kit; P4; Pregn-4-Ene-3; 20-Dione; PG elisa kit

• Reactivity: General
• Specificity: This assay has high sensitivity and excellent specificity for detection of progesterone. No significant cross-reactivity or interference between progesterone and analogues was observed.
• Sequence Length: 933

• Samples: Serum, plasma and other biological fluids
• Assay Type: Quantitative Competitive
• Detection Range: 1.23-100ng/mL
• Sensitivity: < 0.55ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level progesterone were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 sampl es with low, middle and high level progesterone were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PG elisa kit

Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of progesterone in serum, plasma and other biological fluids.

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to progesterone has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled progesterone and unlabeled progesterone (Standards or samples) with the pre-coated antibody specific to progesterone. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of progesterone in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of progesterone in the sample.

NCBI and Uniprot Product Information

• NCBI GI #: 110611914
• NCBI GeneID: 5241
• NCBI Accession #: NP_000917.3
• NCBI GenBank Nucleotide #: NM_000926.4
• UniProt Accession #: P06401; Q8TDS3; Q9UPF7; A7LQ08; A7X8B0; B4E3T0
• Molecular Weight: 87,747 Da
• NCBI Official Full Name: progesterone receptor isoform B
• NCBI Official Synonym Full Names: progesterone receptor
• NCBI Official Symbol: PGR
• NCBI Official Synonym Symbols: PR; NR3C3
• NCBI Protein Information: progesterone receptor
• UniProt Protein Name: Progesterone receptor
• UniProt Gene Name: PGR
• UniProt Synonym Gene Names: NR3C3; PR

NCBI Description

This gene encodes a member of the steroid receptor superfamily. The encoded protein mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. This gene uses two distinct promotors and translation start sites in the first exon to produce several transcript variants, both protein coding and non-protein coding. Two of the isoforms (A and B) are identical except for an additional 165 amino acids found in the N-terminus of isoform B and mediate their own response genes and physiologic effects with little overlap. [provided by RefSeq, Sep 2015]

Uniprot Description

The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Depending on the isoform, progesterone receptor functions as transcriptional activator or repressor.