Rabbit hnRNP H Polyclonal Antibody | anti-hnRNPH antibody

hnRNP H (R192) Polyclonal Antibody

Cat. Num. AAA3002413

• Gene Names: HNRNPH2; FTP3; MRXSB; NRPH2; HNRPH'; HNRPH2; hnRNPH'
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: hnRNP H; Polyclonal Antibody; hnRNP H (R192) Polyclonal Antibody; Heterogeneous nuclear ribonucleoprotein H2; hnRNP H2; FTP-3; Heterogeneous nuclear ribonucleoprotein H'; hnRNP H'; HNRNPH2; FTP3; HNRPH2; anti-hnRNPH antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: hnRNP H (R192) polyclonal antibody detects endogenous levels of hnRNP H protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 449

• Applicable Applications for anti-hnRNPH antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 180-230 of Human hnRNP H.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of hnRNP H (R192) polyclonal antibody at 1:500 dilutionLane1:Hela whole cell lysate (40ug) Lane2:L02 whole cell lysate (40ug) Lane3:C6 whole cell lysate (40ug) Lane4:CT26 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of hnRNP H (R192) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.)

Related Product Information for anti-hnRNPH antibody

Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of polypeptides that contribute to pre-mRNA processing and transport, and also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA polymerase II. The majority of hnRNP proteins components are localized to the nucleus; however some shuttle between the nucleus and the cytoplasm. Most hnRNP proteins, including hnRNP C1 and C2, contain one or more RNA binding domains and are implicated in the processing of pre-mRNA. hnRNPs F and H are largely related factors that preferentially associate with poly(rG) regions on RNA.

NCBI and Uniprot Product Information

• NCBI GI #: 74099697
• NCBI GeneID: 3188
• NCBI Accession #: NP_001027565.1
• NCBI GenBank Nucleotide #: NP_001027565.1
• UniProt Accession #: P55795
• Molecular Weight: ~ 50kDa
• NCBI Official Full Name: heterogeneous nuclear ribonucleoprotein H2
• NCBI Official Synonym Full Names: heterogeneous nuclear ribonucleoprotein H2
• NCBI Official Symbol: HNRNPH2
• NCBI Official Synonym Symbols: FTP3; MRXSB; NRPH2; HNRPH'; HNRPH2; hnRNPH'
• NCBI Protein Information: heterogeneous nuclear ribonucleoprotein H2
• UniProt Protein Name: Heterogeneous nuclear ribonucleoprotein H2
• UniProt Gene Name: HNRNPH2
• UniProt Synonym Gene Names: FTP3; HNRPH2; hnRNP H2; hnRNP H'
• UniProt Entry Name: HNRH2_HUMAN

NCBI Description

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that binds to RNAs. It is very similar to the family member HNRPH1. This gene is thought to be involved in Fabray disease and X-linked agammaglobulinemia phenotype. Alternative splicing results in multiple transcript variants encoding the same protein. Read-through transcription between this locus and the ribosomal protein L36a gene has been observed. [provided by RefSeq, Jan 2011]

Uniprot Description

hnRNP H2: This protein is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Binds poly(RG).

Protein type: RNA splicing; RNA-binding

Chromosomal Location of Human Ortholog: Xq22

Cellular Component: nucleoplasm; membrane; nucleus; ribonucleoprotein complex

Molecular Function: RNA binding; nucleotide binding

Biological Process: nuclear mRNA splicing, via spliceosome; RNA splicing; gene expression