Rabbit anti-Human, Mouse HNMT Polyclonal Antibody | anti-HNMT antibody

HNMT Polyclonal Antibody

Cat. Num. AAA3011019

• Gene Names: HNMT; HMT; MRT51; HNMT-S1; HNMT-S2
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: HNMT; Polyclonal Antibody; HNMT Polyclonal Antibody; Histamine N methyltransferase; Histamine N-methyltransferase; HMT; HNMT S1; HNMT S2; HNMT_HUMAN; anti-HNMT antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: HNMT polyclonal antibody detects endogenous levels of HNMT protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 292

• Applicable Applications for anti-HNMT antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; IP: 1:20-1:50
• Immunogen: Recombinant full length Human HNMT.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of HNMT polyclonal antibody)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analysis of HNMT polyclonal antibody in paraffin-embedded Human Breast Cancer tissue.)

Immunofluorescence (IF)

(Immunofluorescence analysis of U2OS cells, using HNMT polyclonal antibody)

Related Product Information for anti-HNMT antibody

In mammals, histamine is metabolized by two major pathways: N(tau)-methylation via histamine N-methyltransferase and oxidative deamination via diamine oxidase. This gene encodes the first enzyme which is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. In the mammalian brain, the neurotransmitter activity of histamine is controlled by N(tau)-methylation as diamine oxidase is not found in the central nervous system. A common genetic polymorphism affects the activity levels of this gene product in red blood cells.

NCBI and Uniprot Product Information

• NCBI GI #: 1708272
• NCBI GeneID: 3176
• UniProt Accession #: P50135
• Molecular Weight: ~33kDa
• NCBI Official Full Name: Histamine N-methyltransferase
• NCBI Official Synonym Full Names: histamine N-methyltransferase
• NCBI Official Symbol: HNMT
• NCBI Official Synonym Symbols: HMT; MRT51; HNMT-S1; HNMT-S2
• NCBI Protein Information: histamine N-methyltransferase
• UniProt Protein Name: Histamine N-methyltransferase
• Protein Family: Histamine N-methyltransferase
• UniProt Gene Name: HNMT
• UniProt Synonym Gene Names: HMT
• UniProt Entry Name: HNMT_HUMAN

NCBI Description

In mammals, histamine is metabolized by two major pathways: N(tau)-methylation via histamine N-methyltransferase and oxidative deamination via diamine oxidase. This gene encodes the first enzyme which is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. In the mammalian brain, the neurotransmitter activity of histamine is controlled by N(tau)-methylation as diamine oxidase is not found in the central nervous system. A common genetic polymorphism affects the activity levels of this gene product in red blood cells. Multiple alternatively spliced transcript variants that encode different proteins have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

HNMT: Inactivates histamine by N-methylation. Plays an important role in degrading histamine and in regulating the airway response to histamine. Belongs to the methyltransferase superfamily. HNMT family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.1.1.8; Amino Acid Metabolism - histidine; Methyltransferase

Chromosomal Location of Human Ortholog: 2q22.1

Cellular Component: nucleoplasm; neuron projection; cytoplasm

Molecular Function: histamine N-methyltransferase activity

Biological Process: methylation; hyperosmotic response; response to tumor cell; response to glucocorticoid stimulus; respiratory gaseous exchange; brain development; response to cocaine; response to amine stimulus

Disease: Asthma, Susceptibility To