Rabbit anti-Human HNMT Polyclonal Antibody | anti-HNMT antibody

HNMT Antibody

Cat. Num. AAA9402690

• Gene Names: HNMT; HMT; HNMT-S1; HNMT-S2
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: HNMT; Polyclonal Antibody; HNMT Antibody; HMT; HNMT-S1; HNMT-S2; anti-HNMT antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total HNMT protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 51

• Applicable Applications for anti-HNMT antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunohistochemistry: 1:50 - 1:200; Immunofluorescence: 1:50 - 1:200
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human HNMT.
• Target Name: HNMT
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using HNMT antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human thyroid cancer using HNMT antibody at dilution of 1:200 (200x lens).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast cancer using HNMT antibody at dilution of 1:200 (200x lens).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human kidney cancer using HNMT antibody at dilution of 1:200 (400x lens).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human stomach cancer using HNMT antibody at dilution of 1:200 (400x lens).)

Immunofluorescence (IF)

(Immunofluorescence analysis of U2OS cell using HNMT antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-HNMT antibody

In mammals, histamine is metabolized by two major pathways: N(tau)-methylation via histamine N-methyltransferase and oxidative deamination via diamine oxidase. This gene encodes the first enzyme which is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. In the mammalian brain, the neurotransmitter activity of histamine is controlled by N(tau)-methylation as diamine oxidase is not found in the central nervous system. A common genetic polymorphism affects the activity levels of this gene product in red blood cells. Multiple alternatively spliced transcript variants that encode different proteins have been found for this gene.

Product Categories/Family for anti-HNMT antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 66932963
• NCBI GeneID: 3176
• NCBI Accession #: NP_001019245.1
• NCBI GenBank Nucleotide #: NM_001024074.2
• UniProt Accession #: P50135; Q546Z6; Q7Z7I2; Q8IU56; Q8WW98; Q9BRW6; B2R9J3
• Molecular Weight: 6,046 Da
• NCBI Official Full Name: histamine N-methyltransferase isoform 2
• NCBI Official Synonym Full Names: histamine N-methyltransferase
• NCBI Official Symbol: HNMT
• NCBI Official Synonym Symbols: HMT; HNMT-S1; HNMT-S2
• NCBI Protein Information: histamine N-methyltransferase
• UniProt Protein Name: Histamine N-methyltransferase
• Protein Family: Histamine N-methyltransferase
• UniProt Gene Name: HNMT
• UniProt Synonym Gene Names: HMT
• UniProt Entry Name: HNMT_HUMAN

NCBI Description

In mammals, histamine is metabolized by two major pathways: N(tau)-methylation via histamine N-methyltransferase and oxidative deamination via diamine oxidase. This gene encodes the first enzyme which is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. In the mammalian brain, the neurotransmitter activity of histamine is controlled by N(tau)-methylation as diamine oxidase is not found in the central nervous system. A common genetic polymorphism affects the activity levels of this gene product in red blood cells. Multiple alternatively spliced transcript variants that encode different proteins have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

HNMT: Inactivates histamine by N-methylation. Plays an important role in degrading histamine and in regulating the airway response to histamine. Belongs to the methyltransferase superfamily. HNMT family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Methyltransferase; Amino Acid Metabolism - histidine; EC 2.1.1.8

Chromosomal Location of Human Ortholog: 2q22.1

Cellular Component: nucleoplasm; neuron projection; cytoplasm

Molecular Function: histamine N-methyltransferase activity

Biological Process: methylation; hyperosmotic response; response to tumor cell; response to glucocorticoid stimulus; respiratory gaseous exchange; brain development; response to cocaine; response to amine stimulus

Disease: Asthma, Susceptibility To