Western Blot (WB)
(Western blot detection of HNF-4-alpha in K562,C6,Hela cell lysates using HNF-4-alpha Rabbit mAb(1:1000 diluted). Predicted band size:53kDa. Observed band size:53kDa.)
Rabbit HNF-4-alpha Monoclonal Antibody | anti-Hnf4a antibody
HNF-4-alpha Rabbit mAb
Gene Names
Hnf4a; Hnf4; HNF-4; MODY1; Nr2a1; Tcf14; Hnf4alpha
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation
Purity
Affinity purified
Synonyms
HNF-4-alpha; Monoclonal Antibody; HNF-4-alpha Rabbit mAb; Hepatocyte nuclear factor 4-alpha; Hnf4; HNF-4; MODY1; Nr2a1; Tcf14; TCF-14; Hnf4alpha; anti-Hnf4a antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
R01-7D7
Specificity
Expressed in the liver, pancreas and colon in a circadian manner.
Purity/Purification
Affinity purified
Form/Format
Supplied in 50nM Tris-Glycine (pH7.4), 0.15M Nacl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applicable Applications for anti-Hnf4a antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)
Application Notes
WB: 1:1000
IHC: 1:200
IP: 1:20
ChIP: 1:20
IHC: 1:200
IP: 1:20
ChIP: 1:20
Immunogen
Recombinant protein of human HNF-4-alpha
Conjugation
Unconjugated
Modification
Unmodification
Preparation and Storage
Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze/thaw cycle.
Western Blot (WB)
(Western blot detection of HNF-4-alpha in K562,C6,Hela cell lysates using HNF-4-alpha Rabbit mAb(1:1000 diluted). Predicted band size:53kDa. Observed band size:53kDa.)
Western Blot (WB)
(Western blot detection of HNF-4-alpha in K562,C6,Hela cell lysates using HNF-4-alpha Rabbit mAb(1:1000 diluted). Predicted band size:53kDa. Observed band size:53kDa.)
Related Product Information for anti-Hnf4a antibody
The protein encoded by this gene is a transcription factor involved in the development of the pancreas, liver, kidney, and intestines. The encoded protein also functions to maintain glucose homeostasis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2015]
Transcriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1-antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1-alpha. May be essential for development of the liver, kidney and intestine.MiscellaneousBinds fatty acids.
Transcriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1-antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1-alpha. May be essential for development of the liver, kidney and intestine.MiscellaneousBinds fatty acids.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
51,518 Da
NCBI Official Full Name
hepatocyte nuclear factor 4-alpha
NCBI Official Synonym Full Names
hepatic nuclear factor 4, alpha
NCBI Official Symbol
Hnf4a
NCBI Official Synonym Symbols
Hnf4; HNF-4; MODY1; Nr2a1; Tcf14; Hnf4alpha
NCBI Protein Information
hepatocyte nuclear factor 4-alpha
UniProt Protein Name
Hepatocyte nuclear factor 4-alpha
Protein Family
UniProt Gene Name
Hnf4a
UniProt Synonym Gene Names
Hnf-4; Hnf4; Nr2a1; Tcf14; HNF-4-alpha; TCF-14
UniProt Entry Name
HNF4A_MOUSE
NCBI Description
The protein encoded by this gene is a transcription factor involved in the development of the pancreas, liver, kidney, and intestines. The encoded protein also functions to maintain glucose homeostasis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2015]
Research Articles on Hnf4a
Similar Products
Product Notes
The Hnf4a hnf4a (Catalog #AAA4752800) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HNF-4-alpha Rabbit mAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HNF-4-alpha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP). WB: 1:1000 IHC: 1:200 IP: 1:20 ChIP: 1:20. Researchers should empirically determine the suitability of the Hnf4a hnf4a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HNF-4-alpha, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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