Rabbit HMGIY / HMGA1 Polyclonal Antibody | anti-HMGIY / HMGA1 antibody

Anti-HMGIY / HMGA1 Antibody (aa1-53) IHC-plus

Cat. Num. AAA2400483

• Gene Names: HMGA1; HMG-R; HMGIY; HMGA1A
• Reactivity: Mouse, Dog, Rat, Pig, Human
• Applications: Immunohistochemistry, ELISA
• Purity: Immunoaffinity purified
• Synonyms: HMGIY / HMGA1; Polyclonal Antibody; Anti-HMGIY / HMGA1 Antibody (aa1-53) IHC-plus; Rabbit Polyclonal (IgG) to Human HMGIY / HMGA1; Human HMGIY / HMGA1; High mobility group protein A1; High mobility group protein R; HMG-R; Hmg-i; HMGIY; High mobility group AT-hook 1; HMG-I(Y); HMGA1A; anti-HMGIY / HMGA1 antibody

• Host: Rabbit
• Reactivity: Mouse, Dog, Rat, Pig, Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human HMGIY / HMGA1
• Purity/Purification: Immunoaffinity purified
• Form/Format: PBS, pH 7, 1% BSA, 20% Glycerol, 0.01% Thimerosal
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 96

• Applicable Applications for anti-HMGIY / HMGA1 antibody: Immunohistochemistry (IHC) Paraffin, ELISA (EIA)
• Application Notes: IHC-P (10 ug/ml)
• Immunogen: HMGIY / HMGA1 antibody was raised against synthetic peptide corresponding to a region within amino acids 1 and 53 of HMGA1 (SwissProt P17096). Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Pig, Rhesus monkey, Chimpanzee (100%); Bovine (92%).
• Immunogen Description: Synthetic peptide corresponding to a region within amino acids 1 and 53 of HMGA1 (SwissProt P17096). Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Pig, Rhesus monkey, Chimpanzee (100%); Bovine (92%).
• Immunogen Type: Synthetic peptide
• Antigen Modification: aa1-53
• Target Species: Human
• Preparation and Storage: Keep as concentrated solution. Aliquot and store at -20 degree C or below. Avoid multiple freeze-thaw cycles.

Immunohistochemistry (IHC)

(Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE))

Related Product Information for anti-HMGIY / HMGA1 antibody

HMGA1 Antibody, High mobility group protein A1 Antibody, High mobility group protein R Antibody, HMG-R Antibody, Hmg-i Antibody, HMGIY Antibody, High mobility group AT-hook 1 Antibody, HMG-I(Y) Antibody, HMGA1A Antibody Description: HMGIY / HMGA1 is a non-histone protein involved in many cellular processes, including regulation of inducible gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. The encoded protein preferentially binds to the minor groove of A+T-rich regions in double-stranded DNA. It has little secondary structure in solution but assumes distinct conformations when bound to substrates such as DNA or other proteins.

NCBI and Uniprot Product Information

• NCBI GI #: 4504433
• NCBI GeneID: 3159
• NCBI Accession #: NP_002122.1
• NCBI GenBank Nucleotide #: NM_002131.3
• Molecular Weight: 19,694 Da
• NCBI Official Full Name: high mobility group protein HMG-I/HMG-Y isoform b
• NCBI Official Synonym Full Names: high mobility group AT-hook 1
• NCBI Official Symbol: HMGA1
• NCBI Official Synonym Symbols: HMG-R; HMGIY; HMGA1A
• NCBI Protein Information: high mobility group protein HMG-I/HMG-Y
• UniProt Protein Name: High mobility group protein HMG-I/HMG-Y
• UniProt Gene Name: HMGA1
• UniProt Synonym Gene Names: HMGIY; HMG-I(Y); High mobility group protein A1
• UniProt Entry Name: HMGA1_HUMAN

NCBI Description

This gene encodes a chromatin-associated protein involved in the regulation of gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. The encoded protein preferentially binds to the minor groove of AT-rich regions in double-stranded DNA. Multiple transcript variants encoding different isoforms have been found for this gene. Pseudogenes of this gene have been identified on multiple chromosomes. [provided by RefSeq, Jan 2016]

Uniprot Description

HMGA1: HMG-I/Y bind preferentially to the minor groove of A+T rich regions in double stranded DNA. It is suggested that these proteins could function in nucleosome phasing and in the 3'-end processing of mRNA transcripts. They are also involved in the transcription regulation of genes containing, or in close proximity to A+T-rich regions. A chromosomal aberration involving HMGA1 is found in pulmonary chondroid hamartoma. Translocation t(6;14)(p21;q23-24) with RAD51B. Belongs to the HMGA family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 6p21

Cellular Component: cytosol; focal adhesion; nucleoplasm; nucleus; transcription factor complex

Molecular Function: 5'-deoxyribose-5-phosphate lyase activity; AT DNA binding; chromatin binding; DNA binding; DNA-(apurinic or apyrimidinic site) lyase activity; enzyme binding; ligand-dependent nuclear receptor transcription coactivator activity; peroxisome proliferator activated receptor binding; protein binding; retinoic acid receptor binding; retinoid X receptor binding; transcription factor activity; transcription factor binding

Biological Process: base-excision repair; DNA unwinding during replication; negative regulation of cell proliferation; negative regulation of chromatin silencing; negative regulation of transcription, DNA-dependent; nucleosome disassembly; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein complex assembly; regulation of transcription, DNA-dependent; response to virus; transcription from RNA polymerase II promoter; viral reproduction