Rabbit HMGA1 Polyclonal Antibody | anti-HMGA1 antibody

Anti-HMGA1 Antibody

Cat. Num. AAA821312

• Gene Names: HMGA1; HMG-R; HMGIY; HMGA1A
• Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig
• Applications: Western Blot
• Purity: The antibody was purified by immunogen affinity chromatography.
• Synonyms: HMGA1; Polyclonal Antibody; Anti-HMGA1 Antibody; HMGIY; High mobility group protein HMG-I/HMG-Y; HMG-I(Y); High mobility group AT-hook protein 1; High mobility group protein A1; High mobility group protein R; anti-HMGA1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig
• Clonality: Polyclonal
• Specificity: Recognizes endogenous levels of HMGA1 protein.
• Purity/Purification: The antibody was purified by immunogen affinity chromatography.
• Form/Format: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Sequence Length: 96

• Applicable Applications for anti-HMGA1 antibody: Western Blot (WB)
• Application Notes: WB (1/500 - 1/1000)
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human HMGA1. The exact sequence is proprietary.
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of HMGA1 expression in Hela (A), Raw264.7 (B), NIH3T3 (C) whole cell lysates.)

Related Product Information for anti-HMGA1 antibody

Rabbit polyclonal antibody to HMGA1

NCBI and Uniprot Product Information

• NCBI GI #: 4504433
• NCBI GeneID: 3159
• NCBI Accession #: NP_002122.1
• NCBI GenBank Nucleotide #: NM_002131.3
• UniProt Accession #: P17096; P10910; Q5T6U9; Q9UKB0
• Molecular Weight: 19,694 Da
• NCBI Official Full Name: high mobility group protein HMG-I/HMG-Y isoform b
• NCBI Official Synonym Full Names: high mobility group AT-hook 1
• NCBI Official Symbol: HMGA1
• NCBI Official Synonym Symbols: HMG-R; HMGIY; HMGA1A
• NCBI Protein Information: high mobility group protein HMG-I/HMG-Y; HMG-I(Y); high mobility group protein R; high mobility group protein A1; nonhistone chromosomal high-mobility group protein HMG-I/HMG-Y; high-mobility group (nonhistone chromosomal) protein isoforms I and Y
• UniProt Protein Name: High mobility group protein HMG-I/HMG-Y
• Protein Family: High mobility group protein
• UniProt Gene Name: HMGA1
• UniProt Synonym Gene Names: HMGIY; HMG-I(Y); High mobility group protein A1
• UniProt Entry Name: HMGA1_HUMAN

NCBI Description

This gene encodes a non-histone protein involved in many cellular processes, including regulation of inducible gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. The encoded protein preferentially binds to the minor groove of A+T-rich regions in double-stranded DNA. It has little secondary structure in solution but assumes distinct conformations when bound to substrates such as DNA or other proteins. The encoded protein is frequently acetylated and is found in the nucleus. At least seven transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

HMGA1: HMG-I/Y bind preferentially to the minor groove of A+T rich regions in double stranded DNA. It is suggested that these proteins could function in nucleosome phasing and in the 3'-end processing of mRNA transcripts. They are also involved in the transcription regulation of genes containing, or in close proximity to A+T-rich regions. A chromosomal aberration involving HMGA1 is found in pulmonary chondroid hamartoma. Translocation t(6;14)(p21;q23-24) with RAD51B. Belongs to the HMGA family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 6p21

Cellular Component: nucleoplasm; transcription factor complex; focal adhesion; nucleus; cytosol

Molecular Function: retinoid X receptor binding; protein binding; peroxisome proliferator activated receptor binding; enzyme binding; DNA-(apurinic or apyrimidinic site) lyase activity; ligand-dependent nuclear receptor transcription coactivator activity; DNA binding; AT DNA binding; retinoic acid receptor binding; 5'-deoxyribose-5-phosphate lyase activity; transcription factor binding

Biological Process: DNA unwinding during replication; transcription, DNA-dependent; viral reproduction; response to virus; positive regulation of transcription, DNA-dependent; DNA catabolic process, endonucleolytic; negative regulation of cell proliferation; regulation of transcription, DNA-dependent; nucleosome disassembly; negative regulation of chromatin silencing; base-excision repair; protein complex assembly; negative regulation of transcription, DNA-dependent