Western Blot (WB)
(All lanes : Anti-Hmga2 Antibody (C-term) at 1:1000 dilutionLane 1: F9 whole cell lysatesLane 2: HepG2 whole cell lysatesLane 3: NCCIT whole cell lysatesLane 4: NIH/3T3 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 12 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human, Mouse Hmga2 Polyclonal Antibody | anti-Hmga2 antibody
Mouse Hmga2 Antibody (C-term)
Gene Names
Hmga2; pg; Hmgic; pygmy; HMGI-C; 9430083A20Rik
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence, ELISA
Synonyms
Hmga2; Polyclonal Antibody; Mouse Hmga2 Antibody (C-term); High mobility group protein HMGI-C; High mobility group AT-hook protein 2; Hmgic; anti-Hmga2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
Ig
Specificity
This Mouse Hmga2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 72-105 amino acids from the C-terminal region of Mouse Hmga2.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
108
Applicable Applications for anti-Hmga2 antibody
Western Blot (WB), Immunofluorescence (IF), ELISA( EIA)
Application Notes
WB: 1:1000
IF: 1:25
IF: 1:25
Antigen Source
Human
Antigen Region
72-105
Antigen Type
Synthetic Peptide
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(All lanes : Anti-Hmga2 Antibody (C-term) at 1:1000 dilutionLane 1: F9 whole cell lysatesLane 2: HepG2 whole cell lysatesLane 3: NCCIT whole cell lysatesLane 4: NIH/3T3 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 12 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-Hmga2 Antibody (C-term) at 1:1000 dilutionLane 1: F9 whole cell lysatesLane 2: HepG2 whole cell lysatesLane 3: NCCIT whole cell lysatesLane 4: NIH/3T3 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 12 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Immunofluorescence (IF)
(Fluorescent image of C2C12cells stained with Mouse Hmga2 Antibody (C-term) . It was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Immunofluorescence (IF)
(Fluorescent image of C2C12cells stained with Mouse Hmga2 Antibody (C-term) . It was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Related Product Information for anti-Hmga2 antibody
Description: Purified Rabbit Polyclonal Antibody (Pab)
Background: Functions as a transcriptional regulator. Functions in cell cycle regulation through CCNA2. Plays an important role in chromosome condensation during the meiotic G2/M transition of spermatocytes.
Background: Functions as a transcriptional regulator. Functions in cell cycle regulation through CCNA2. Plays an important role in chromosome condensation during the meiotic G2/M transition of spermatocytes.
Product Categories/Family for anti-Hmga2 antibody
References
Manfioletti G.,et al.Nucleic Acids Res. 19:6793-6797(1991). Manfioletti G.,et al.Gene 167:249-253(1995). Zhou X.,et al.Nucleic Acids Res. 24:4071-4077(1996). Carninci P.,et al.Science 309:1559-1563(2005). Di Agostino S.,et al.Mol. Biol. Cell 15:1224-1232(2004).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
11819
NCBI Official Full Name
high mobility group protein HMGI-C isoform 1
NCBI Official Synonym Full Names
high mobility group AT-hook 2
NCBI Official Symbol
Hmga2
NCBI Official Synonym Symbols
pg; Hmgic; pygmy; HMGI-C; 9430083A20Rik
NCBI Protein Information
high mobility group protein HMGI-C
UniProt Protein Name
High mobility group protein HMGI-C
Protein Family
UniProt Gene Name
Hmga2
UniProt Synonym Gene Names
Hmgic
UniProt Entry Name
HMGA2_MOUSE
Research Articles on Hmga2
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Product Notes
The Hmga2 hmga2 (Catalog #AAA9231949) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Mouse Hmga2 Antibody (C-term) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Hmga2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), ELISA( EIA). WB: 1:1000 IF: 1:25. Researchers should empirically determine the suitability of the Hmga2 hmga2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hmga2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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