Western Blot (WB)
(Western blot analysis of extracts from LOVO cells, using HLAH antibody.)
Rabbit anti-Human HLAH Polyclonal Antibody | anti-HLAH antibody
HLAH Antibody
Gene Names
HLA-H; HLAHP
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
HLAH; Polyclonal Antibody; HLAH Antibody; HLA-12.4; HLA-AR; HLA-H; HLAHP; Major histocompatibility complex; class I; H (pseudogene); MHC class I antigen H; Putative HLA class I histocompatibility antigen; alpha chain H; anti-HLAH antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
HLAH antibody detects endogenous levels of total HLAH
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
362
Applicable Applications for anti-HLAH antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Subcellular Location
Cell Membrane; Single-Pass Membrane Protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from LOVO cells, using HLAH antibody.)
Western Blot (WB)
(Western blot analysis of extracts from LOVO cells, using HLAH antibody.)
Immunofluorescene (IF)
(MBS9605572 staining LOVO cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Immunofluorescene (IF)
(MBS9605572 staining LOVO cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Related Product Information for anti-HLAH antibody
Function: Involved in the presentation of foreign antigens to the immune system.
Subunit Structure: Heterodimer of an alpha chain and a beta chain (beta-2-microglobulin).
Similarity: Belongs to the MHC class I family.
Subunit Structure: Heterodimer of an alpha chain and a beta chain (beta-2-microglobulin).
Similarity: Belongs to the MHC class I family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Observed: 40 kD
Predicted: 41 kDa
Predicted: 41 kDa
NCBI Official Full Name
Putative HLA class I histocompatibility antigen, alpha chain H
NCBI Official Synonym Full Names
major histocompatibility complex, class I, H (pseudogene)
NCBI Official Symbol
HLA-H
NCBI Official Synonym Symbols
HLAHP
UniProt Protein Name
Putative HLA class I histocompatibility antigen, alpha chain H
UniProt Gene Name
HLA-H
UniProt Synonym Gene Names
HLAH
NCBI Description
This major histocompatibility complex gene represents a transcribed pseudogene, possibly derived from HLA-A. This gene displays extensive variation. [provided by RefSeq, May 2010]
Uniprot Description
Involved in the presentation of foreign antigens to the immune system.CautionCould be the product of a pseudogene.
Research Articles on HLAH
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Product Notes
The HLAH hla-h (Catalog #AAA9605572) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HLAH Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HLAH can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the HLAH hla-h for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HLAH, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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