Human Cytochrome P450 1A1 ELISA Kit | CYP1A1 elisa kit

Human Cytochrome P450 1A1 ELISA Kit

Cat. Num. AAA761788

• Gene Names: CYP1A1; AHH; AHRR; CP11; CYP1; CYPIA1; P1-450; P450-C; P450DX
• Reactivity: Human
• Synonyms: Cytochrome P450 1A1; Human Cytochrome P450 1A1 ELISA Kit; CYP1A1 (Cytochrome P450; family 1; subfamily A; polypeptide 1)/Cytochrome P450 1A1/Cytochrome P450-P1/Cytochrome P450-C/CYPIA1/Cytochrome P450 form 6; CYP1A1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CYP1A1. No significant cross-reactivity or interference between CYP1A1 and analogues was observed.
• Sequence Length: 512

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for CYP1A1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4503199
• NCBI GeneID: 1543
• NCBI Accession #: NP_000490.1
• NCBI GenBank Nucleotide #: NM_000499.4
• Molecular Weight: 18,441 Da
• NCBI Official Full Name: cytochrome P450 1A1 isoform 1
• NCBI Official Synonym Full Names: cytochrome P450 family 1 subfamily A member 1
• NCBI Official Symbol: CYP1A1
• NCBI Official Synonym Symbols: AHH; AHRR; CP11; CYP1; CYPIA1; P1-450; P450-C; P450DX
• NCBI Protein Information: cytochrome P450 1A1
• UniProt Protein Name: Cytochrome P450 1A1
• Protein Family: Cytochrome
• UniProt Gene Name: CYP1A1
• UniProt Entry Name: CP1A1_HUMAN

NCBI Description

This gene, CYP1A1, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. The gene has been associated with lung cancer risk. A related family member, CYP1A2, is located approximately 25 kb away from CYP1A1 on chromosome 15. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jan 2016]

Uniprot Description

CYP1A1: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Belongs to the cytochrome P450 family.

Protein type: Amino Acid Metabolism - tryptophan; Xenobiotic Metabolism - metabolism by cytochrome P450; Cofactor and Vitamin Metabolism - retinol; Oxidoreductase; EC 1.14.14.1

Chromosomal Location of Human Ortholog: 15q24.1

Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; mitochondrion

Molecular Function: demethylase activity; enzyme binding; flavonoid 3'-monooxygenase activity; heme binding; iron ion binding; monooxygenase activity; oxidoreductase activity; oxidoreductase activity, acting on diphenols and related substances as donors; oxygen binding; protein binding; steroid hydroxylase activity

Biological Process: 9-cis-retinoic acid biosynthetic process; aging; amine metabolic process; arachidonic acid metabolic process; camera-type eye development; cell proliferation; cellular lipid metabolic process; coumarin metabolic process; dibenzo-p-dioxin catabolic process; drug metabolic process; embryonic development ending in birth or egg hatching; epoxygenase P450 pathway; flavonoid metabolic process; gut development; hydrogen peroxide biosynthetic process; insecticide metabolic process; maternal process involved in parturition; porphyrin metabolic process; response to antibiotic; response to arsenic; response to drug; response to food; response to herbicide; response to hyperoxia; response to hypoxia; response to iron(III) ion; response to lipopolysaccharide; response to nematode; response to virus; response to vitamin A; response to wounding; vitamin D metabolic process; xenobiotic metabolic process