Rabbit anti-Human, Mouse HLA-F Polyclonal Antibody | anti-HLA-F antibody

HLA-F Rabbit Polyclonal

Cat. Num. AAA7600145

• Gene Names: HLA-F; HLAF; CDA12; HLA-5.4; HLA-CDA12
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: HLA-F; Polyclonal Antibody; HLA-F Rabbit Polyclonal; anti-HLA-F antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 442

• Applicable Applications for anti-HLA-F antibody: ELISA (EIA), Western Blot (WB)
• Immunogen: Major histocompatibility complex, class I, F
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Western Blot (WB)

(Raji cells were subjected to SDS PAGE followed by western blot with MBS7600145 (HLA-F antibody) at dilution of 1:2000)

NCBI and Uniprot Product Information

• NCBI GI #: 149158702
• NCBI GeneID: 3134
• NCBI Accession #: NP_001091949.1
• NCBI GenBank Nucleotide #: NM_001098479.1
• Molecular Weight: 50,438 Da
• NCBI Official Full Name: HLA class I histocompatibility antigen, alpha chain F isoform 1
• NCBI Official Synonym Full Names: major histocompatibility complex, class I, F
• NCBI Official Symbol: HLA-F
• NCBI Official Synonym Symbols: HLAF; CDA12; HLA-5.4; HLA-CDA12
• NCBI Protein Information: HLA class I histocompatibility antigen, alpha chain F
• UniProt Protein Name: HLA class I histocompatibility antigen, alpha chain F
• Protein Family: HLA class I histocompatibility antigen
• UniProt Gene Name: HLA-F
• UniProt Synonym Gene Names: HLA-5.4; HLAF
• UniProt Entry Name: HLAF_HUMAN

NCBI Description

This gene belongs to the HLA class I heavy chain paralogues. It encodes a non-classical heavy chain that forms a heterodimer with a beta-2 microglobulin light chain, with the heavy chain anchored in the membrane. Unlike most other HLA heavy chains, this molecule is localized in the endoplasmic reticulum and Golgi apparatus, with a small amount present at the cell surface in some cell types. It contains a divergent peptide-binding groove, and is thought to bind a restricted subset of peptides for immune presentation. This gene exhibits few polymorphisms. Multiple transcript variants encoding different isoforms have been found for this gene. These variants lack a coding exon found in transcripts from other HLA paralogues due to an altered splice acceptor site, resulting in a shorter cytoplasmic domain. [provided by RefSeq, Jul 2008]

Uniprot Description

HLA-F: Involved in the presentation of foreign antigens to the immune system. Belongs to the MHC class I family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: cell surface; early endosome membrane; endoplasmic reticulum; Golgi membrane; membrane; MHC class I protein complex; phagocytic vesicle membrane; plasma membrane

Molecular Function: peptide antigen binding; receptor binding; TAP1 binding; TAP2 binding

Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; antigen processing and presentation of peptide antigen via MHC class I; regulation of immune response