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Western Blot (WB) (Western Blot: Sample: Recombinant F1+2, Human)

Mouse anti-Human Prothrombin Fragment 1+2 (F1+2) Monoclonal Antibody | anti-F1+2 antibody

Monoclonal Antibody to Prothrombin Fragment 1+2 (F1+2)

Gene Names
F2; PT; THPH1; RPRGL2
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Protein A + Protein G affinity chromatography
Synonyms
Prothrombin Fragment 1+2 (F1+2); Monoclonal Antibody; Monoclonal Antibody to Prothrombin Fragment 1+2 (F1+2); anti-F1+2 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1 Kappa
Clone Number
C6
Purity/Purification
Protein A + Protein G affinity chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Concentration
1mg/mL (varies by lot)
Applicable Applications for anti-F1+2 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
Western blotting: 0.5-3ug/mL
Immunohistochemistry: 5-30ug/mL
Immunocytochemistry: 5-30ug/mL
Optimal working dilutions must be determined by end user.
Organism Species
Homo sapiens (Human)
Source
Monoclonal antibody preparation
Traits
Liquid
Immunogen
Recombinant F1+2 expressed in E.coli (MBS2030714)
Cross Reactivity
Human
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 24 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot: Sample: Recombinant F1+2, Human)

Western Blot (WB) (Western Blot: Sample: Recombinant F1+2, Human)

Western Blot (WB)

(Western Blot; Sample: Human Serum;Primary Ab: 2ug/ml Mouse Anti-Human F1 2 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody(Catalog: MBS2086035))

Western Blot (WB) (Western Blot; Sample: Human Serum;Primary Ab: 2ug/ml Mouse Anti-Human F1 2 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody(Catalog: MBS2086035))

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Liver Tissue;Primary Ab: 20ug/ml Mouse Anti-Human F1 2 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody(Catalog: MBS2086035))

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Human Liver Tissue;Primary Ab: 20ug/ml Mouse Anti-Human F1 2 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody(Catalog: MBS2086035))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
prothrombin isoform 1 preproprotein
NCBI Official Synonym Full Names
coagulation factor II, thrombin
NCBI Official Symbol
F2
NCBI Official Synonym Symbols
PT; THPH1; RPRGL2
NCBI Protein Information
prothrombin
UniProt Protein Name
Prothrombin
UniProt Gene Name
F2

NCBI Description

Coagulation factor II is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life. Peptides derived from the C-terminus of this protein have antimicrobial activity against E. coli and P. aeruginosa. Mutations in F2 lead to various forms of thrombosis and dysprothrombinemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]

Uniprot Description

Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing.

Research Articles on F1+2

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Product Notes

The F1+2 f2 (Catalog #AAA2090567) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to Prothrombin Fragment 1+2 (F1+2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Prothrombin Fragment 1+2 (F1+2) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). Western blotting: 0.5-3ug/mL Immunohistochemistry: 5-30ug/mL Immunocytochemistry: 5-30ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the F1+2 f2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Prothrombin Fragment 1+2 (F1+2), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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