Rabbit anti-Human HLA class II histocompatibility antigen, DQ alpha 1 chain Polyclonal Antibody | anti-HLA-DPA1 antibody

HLA class II histocompatibility antigen, DQ alpha 1 chain Polyclonal Antibody

Cat. Num. AAA9415633

• Gene Names: HLA-DPA1; PLT1; HLADP; HLASB; DP(W3); DP(W4); HLA-DP1A
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Caprylic Acid Ammonium Sulfate Precipitation purified
• Synonyms: HLA class II histocompatibility antigen; DQ alpha 1 chain; Polyclonal Antibody; DQ alpha 1 chain Polyclonal Antibody; DC-1 alpha chain; DC-alpha; HLA-DCA; MHC class II DQA1; anti-HLA-DPA1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total HLA class II histocompatibility antigen, DQ alpha 1 chain polyclonal antibody.
• Purity/Purification: Caprylic Acid Ammonium Sulfate Precipitation purified
• Form/Format: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 260

• Applicable Applications for anti-HLA-DPA1 antibody: Immunohistochemistry (IHC)
• Application Notes: Immunohistochemistry: 1:20 - 1:200
• Immunogen: Recombinant Human HLA class II histocompatibility antigen, DQ alpha 1 chain
• Immunogen Type: Protein
• Preparation and Storage: Store at -20 degree C

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embeded human prostate using at dilution of 1:50.)

Related Product Information for anti-HLA-DPA1 antibody

Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.

Product Categories/Family for anti-HLA-DPA1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 335883185
• NCBI GeneID: 3113
• NCBI Accession #: NP_001229453.1
• NCBI GenBank Nucleotide #: NM_001242524.1
• UniProt Accession #: P20036; O19722; O46883; P01905; P79554; Q2Q060; Q2Q061; Q5EY03; Q5STP1; Q6DQK4; A9YWH7; B9UKH4
• Molecular Weight: 29,381 Da
• NCBI Official Full Name: HLA class II histocompatibility antigen, DP alpha 1 chain
• NCBI Official Synonym Full Names: major histocompatibility complex, class II, DP alpha 1
• NCBI Official Symbol: HLA-DPA1
• NCBI Official Synonym Symbols: PLT1; HLADP; HLASB; DP(W3); DP(W4); HLA-DP1A
• NCBI Protein Information: HLA class II histocompatibility antigen, DP alpha 1 chain
• UniProt Protein Name: HLA class II histocompatibility antigen, DP alpha 1 chain
• Protein Family: HLA class II histocompatibility antigen
• UniProt Gene Name: HLA-DPA1
• UniProt Synonym Gene Names: HLA-DP1A; HLASB
• UniProt Entry Name: DPA1_HUMAN

Uniprot Description

HLA-DPA1: Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading. Belongs to the MHC class II family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: cell surface; Golgi membrane; lysosomal membrane; MHC class II protein complex; plasma membrane; trans-Golgi network membrane

Molecular Function: peptide antigen binding

Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; positive regulation of interferon-gamma production; positive regulation of T cell activation; positive regulation of T cell proliferation; T cell costimulation; T cell receptor signaling pathway