Rabbit anti-Human HA2Q Polyclonal Antibody | anti-HA2Q antibody

HA2Q Antibody

Cat. Num. AAA9408031

• Gene Names: HLA-DPA1; PLT1; HLADP; HLASB; DP(W3); DP(W4); HLA-DP1A
• Reactivity: Human
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: HA2Q; Polyclonal Antibody; HA2Q Antibody; DP(W3); DP(W4); DPA1; HLA class II histocompatibility antigen; DP alpha 1 chain; anti-HA2Q antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total HA2Q protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 260

• Applicable Applications for anti-HA2Q antibody: Western Blot (WB)
• Application Notes: Western blotting: 1:500~1:3000
• Immunogen Type: Peptide
• Immunogen Description: Synthesized peptide derived from N-terminal of human HA2Q.
• Target Name: HA2Q
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts from Jurkat cells, using HA2Q antibody.)

Related Product Information for anti-HA2Q antibody

Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal microenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.Lawrance S.K., Nucleic Acids Res. 13:7515-7528(1985).Gustafsson K., J. Biol. Chem. 262:8778-8786(1987).Young J.A., Hum. Immunol. 23:37-44(1988).

Product Categories/Family for anti-HA2Q antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 335883185
• NCBI GeneID: 3113
• NCBI Accession #: NP_001229453.1
• NCBI GenBank Nucleotide #: NM_001242524.1
• UniProt Accession #: P20036; O19722; O46883; P01905; P79554; Q2Q060; Q2Q061; Q5EY03; Q5STP1; Q6DQK4; A9YWH7; B9UKH4
• Molecular Weight: 29,381 Da
• NCBI Official Full Name: HLA class II histocompatibility antigen, DP alpha 1 chain
• NCBI Official Synonym Full Names: major histocompatibility complex, class II, DP alpha 1
• NCBI Official Symbol: HLA-DPA1
• NCBI Official Synonym Symbols: PLT1; HLADP; HLASB; DP(W3); DP(W4); HLA-DP1A
• NCBI Protein Information: HLA class II histocompatibility antigen, DP alpha 1 chain
• UniProt Protein Name: HLA class II histocompatibility antigen, DP alpha 1 chain
• UniProt Gene Name: HLA-DPA1
• UniProt Synonym Gene Names: HLA-DP1A; HLASB
• UniProt Entry Name: DPA1_HUMAN

NCBI Description

HLA-DPA1 belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta (DPB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DP molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to 4 different molecules. [provided by RefSeq, Jul 2008]

Uniprot Description

HLA-DPA1: Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading. Belongs to the MHC class II family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: Golgi membrane; cell surface; lysosomal membrane; integral to plasma membrane; plasma membrane; trans-Golgi network membrane; endosome membrane; MHC class II protein complex

Molecular Function: MHC class II receptor activity; peptide antigen binding

Biological Process: positive regulation of interferon-gamma production; cytokine and chemokine mediated signaling pathway; T cell costimulation; antigen processing and presentation of exogenous peptide antigen via MHC class II; positive regulation of T cell proliferation; immune response; positive regulation of T cell activation; T cell receptor signaling pathway