Rabbit anti-Human HLA Class 2 Antigen DRA Polyclonal Antibody | anti-HLA-DRA antibody

HLA Class 2 Antigen DRA, NT (HLA Class II Histocompatibility Antigen DR alpha Chain, HLA-DRA, HLA-DRA1, FLJ51114, MHC Class II Antigen DRA, MLRW) (HRP)

Cat. Num. AAA6480957

• Gene Names: HLA-DRA; HLA-DRA1
• Reactivity: Human
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: HLA Class 2 Antigen DRA; Polyclonal Antibody; NT (HLA Class II Histocompatibility Antigen DR alpha Chain; HLA-DRA; HLA-DRA1; FLJ51114; MHC Class II Antigen DRA; MLRW) (HRP); Major Histocompatibility Complex; Class II; DR alpha; anti-HLA-DRA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HLA-DRA at ~29kD
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-HLA-DRA antibody: Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FC/FACS: 1:10-1:50; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids aa54-84 in the N-terminal region of human HLA-DRA, UniProt Accession #NP_061984, P01903 Cellular Localization: Cell membrane
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

Western Blot (WB)

(Western blot analysis of HLA-DRA Antibody (N-term) in Ramos cell line lysates (35ug/lane). HLA-DRA (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(HLA-DRA Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HLA-DRA Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(HLA-DRA Antibody (N-term) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-HLA-DRA antibody

Antibodies; HLA

References

1. De Jager, et.al., Nat. Genet. 41(7), 776-782 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 52426774
• NCBI GeneID: 3122
• NCBI Accession #: NP_061984
• NCBI GenBank Nucleotide #: NM_019111.4
• UniProt Accession #: P01903; Q30160; Q6IAZ1; Q861I2; Q9TP70; A2BET4
• Molecular Weight: 24.3 kDa (212aa) confirmed by MALDI-TOF
• NCBI Official Full Name: HLA class II histocompatibility antigen, DR alpha chain
• NCBI Official Synonym Full Names: major histocompatibility complex, class II, DR alpha
• NCBI Official Symbol: HLA-DRA
• NCBI Official Synonym Symbols: HLA-DRA1
• NCBI Protein Information: HLA class II histocompatibility antigen, DR alpha chain
• UniProt Protein Name: HLA class II histocompatibility antigen, DR alpha chain
• Protein Family: HLA class II histocompatibility antigen
• UniProt Gene Name: HLA-DRA
• UniProt Synonym Gene Names: HLA-DRA1
• UniProt Entry Name: DRA_HUMAN

NCBI Description

HLA-DRA is one of the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5. [provided by RefSeq, Jul 2008]

Uniprot Description

HLA-DRA: Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading. Belongs to the MHC class II family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: Golgi membrane; cell surface; lysosome; lysosomal membrane; late endosome membrane; integral to plasma membrane; plasma membrane; trans-Golgi network membrane; MHC class II protein complex

Molecular Function: MHC class II receptor activity; peptide antigen binding

Biological Process: peptide antigen assembly with MHC class II protein complex; cytokine and chemokine mediated signaling pathway; T cell costimulation; antigen processing and presentation of exogenous peptide antigen via MHC class II; immune response; cognition; T cell receptor signaling pathway; antigen processing and presentation of peptide or polysaccharide antigen via MHC class II; polysaccharide assembly with MHC class II protein complex

Disease: Hepatitis B Virus, Susceptibility To