Rabbit Histone H4 Polyclonal Antibody | anti-HIST1H4A antibody

Anti-Histone H4 (TriMethyl K79) Methyl Antibody

Cat. Num. AAA8242453

• Gene Names: HIST2H4A; H4; H4/n; H4F2; H4FN; FO108; HIST2H4
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was purified by affinity chromatography.
• Synonyms: Histone H4; Polyclonal Antibody; Anti-Histone H4 (TriMethyl K79) Methyl Antibody; H4/A; H4FA; H4/I; H4FI; H4/G; H4FG; H4/B; H4FB; H4/J; H4FJ; H4/C; H4FC; H4/H; H4FH; H4/M; H4FM; H4/E; H4FE; H4/D; H4FD; H4/K; H4FK; H4/N; H4F2; H4FN; HIST2H4; H4/O; H4FO; anti-HIST1H4A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Recognizes endogenous levels of Histone H4 (TriMethyl K79) protein.
• Purity/Purification: The antibody was purified by affinity chromatography.
• Form/Format: Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.
• Sequence Length: 103

• Applicable Applications for anti-HIST1H4A antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western Blot: (1/500 - 1/1000); Immunohistochemistry: (1/100 - 1/200)
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of human Histone H4. The exact sequence is proprietary.
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of Histone H4 (TriMethyl K79) expression in Hela (A), NIH3T3 (B) whole cell lysates.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of Histone H4 (TriMethyl K79) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

Related Product Information for anti-HIST1H4A antibody

Rabbit polyclonal to Histone H4 (TriMethyl K79)

NCBI and Uniprot Product Information

• NCBI GI #: 51317339
• NCBI GeneID: 8370
• UniProt Accession #: P62805; P02304; P02305; Q6DRA9; Q6FGB8; Q6NWP7; A2VCL0
• Molecular Weight: 11,367 Da
• NCBI Official Full Name: Histone H4
• NCBI Official Synonym Full Names: histone cluster 2, H4a
• NCBI Official Symbol: HIST2H4A
• NCBI Official Synonym Symbols: H4; H4/n; H4F2; H4FN; FO108; HIST2H4
• NCBI Protein Information: histone H4
• UniProt Protein Name: Histone H4
• Protein Family: Histone
• UniProt Gene Name: HIST1H4A
• UniProt Synonym Gene Names: H4/A; H4FA
• UniProt Entry Name: H4_HUMAN

NCBI Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy. [provided by RefSeq, Aug 2015]

Uniprot Description

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.