MCSF recombinant protein

MCSF, 33-190aa, Human, His tag, E Coli

Cat. Num. AAA203491

• Gene Names: CSF1; MCSF; CSF-1
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: MCSF; 33-190aa; Human; His tag; E Coli; Macrophage colony stimulating factor; CSF1; Macrophage colony stimulating factor Colony stimulating factor 1; CSF 1; Lanimostim; M CSF; Macrophage Colony Stimulating Factor 1; MGC31930.; MCSF recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20 mM Tris-HCl Buffer (pH 8.0), 2mM DTT, 10% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MEEVSEYCSH MIGSGHLQSL QRLIDSQMET SCQITFEFVD QEQLKDPVCY LKKAFLLVQD IMEDTMRFRD NTPNAIAIVQ LQELSLRLKS CFTKDYEEHD KACVRTFYET PLQLLEKVKN VFNETKNLLD KDWNIFSKNC NNSFAECSSQ DVVTKPDCN
• Sequence Length: 554

• Applicable Applications for MCSF recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for MCSF recombinant protein

Macrophage colony stimulating factor (M-CSF), as known as CSF-1, one of the hematopoietic growth factors that regulate the growth and differentiation of blood cells. This protein is produced by monocytes, granulocytes, endothelial cells, and fibroblasts. It stimulates the formation of macrophage colonies, enhances antibody-dependent, cell-mediated cytotoxicity by monocytes and macrophages, and inhibits bone resorption by osteoclasts. Recombinant human M-CSF, fused to His-tag at N-terminus, was expressed as insoluble protein aggregate in E Coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer..

Product Categories/Family for MCSF recombinant protein

Hormones & Cytokines

References

Wei S., et al. (2006). J Leukoc Biol. 80 (6):1445-53.; Woo KM., et al. (2002). Exp Mol Med. 34 (5):340-6.;

NCBI and Uniprot Product Information

• NCBI GI #: 27262667
• NCBI GeneID: 1435
• NCBI Accession #: NP_757351
• NCBI GenBank Nucleotide #: NM_172212.2
• UniProt Accession #: P09603; Q13130; Q14086; Q14806; Q5VVF3; Q5VVF4; Q9UQR8; A8K6J5
• Molecular Weight: 20.7 kDa (179aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: macrophage colony-stimulating factor 1 isoform a
• NCBI Official Synonym Full Names: colony stimulating factor 1
• NCBI Official Symbol: CSF1
• NCBI Official Synonym Symbols: MCSF; CSF-1
• NCBI Protein Information: macrophage colony-stimulating factor 1
• UniProt Protein Name: Macrophage colony-stimulating factor 1
• UniProt Gene Name: CSF1
• UniProt Synonym Gene Names: CSF-1; M-CSF; MCSF

NCBI Description

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. The encoded protein may be involved in development of the placenta. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011]

Uniprot Description

Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance.