Chicken insulin ELISA Kit | INS elisa kit

Chicken Insulin ELISA Kit

Cat. Num. AAA701713

• Reactivity: Chicken
• Synonyms: insulin; Chicken Insulin ELISA Kit; Chicken Insulin ELISA kit; ILPR; IRDN; OTTHUMP00000011162; OTTHUMP00000196038; proinsulin; INS elisa kit

• Reactivity: Chicken
• Specificity: This assay has high sensitivity and excellent specificity for detection of fish INS. No significant cross-reactivity or interference between fish INS and analogues was observed.
• Sequence Length: 107

• Samples: Serum, Plasma, Tissue Homogenates
• Assay Type: Competitive
• Detection Range: 100 pg/ml-1600 pg/ml
• Sensitivity: 100 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for INS elisa kit

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to INS. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated INS. A competitive inhibition reaction is launched between INS (Standards or samples) and Biotin-conjugated INS with the pre-coated antibody specific for INS. The more amount of INS in samples, the less antibody bound by Biotin-conjugated INS. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of INS in the sample. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 45382573
• NCBI GeneID: 396145
• NCBI Accession #: NP_990553.1
• NCBI GenBank Nucleotide #: NM_205222.3
• UniProt Accession #: P67970; P01332; Q53YX4
• Molecular Weight: 11,981 Da
• NCBI Official Full Name: insulin preproinsulin
• NCBI Official Synonym Full Names: insulin
• NCBI Official Symbol: INS
• NCBI Protein Information: insulin
• UniProt Protein Name: Insulin
• Protein Family: Insulin
• UniProt Gene Name: INS
• UniProt Entry Name: INS_CHICK

Uniprot Description

Function: Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.