Mouse anti-Human CD36 Monoclonal Antibody | anti-CD36 antibody

Anti-Hu CD36 Biotin

Cat. Num. AAA1801876

• Gene Names: CD36; FAT; GP4; GP3B; GPIV; CHDS7; PASIV; SCARB3; BDPLT10
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: The purified antibody is conjugated with biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
• Synonyms: CD36; Monoclonal Antibody; Anti-Hu CD36 Biotin; GPIIIb; GPIV; PAS-4; FAT; Thrombospondin receptor; PASIV; CD36 molecule; anti-CD36 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: TR9
• Specificity: The antibody TR9 reacts with an extracellular epitope of CD36 (GPIIIb), a 85kDa integral membrane glycoprotein expressed on platelets, macrophages, endothelial cells, early erythroid cells and megakaryocytes. The antibody TR9 cross-blocks binding of FITC-labeled standard antibody OKM5. Anti-CD36 antibodies inhibit adhesive Functional Application (FUNC)tions (e.g. adherence of infected erythrocytes to target cells).
• Purity/Purification: The purified antibody is conjugated with biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
• Form/Format: Phosphate buffered saline (PBS) solution with 15mM sodium azide
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CD36 antibody: Flow Cytometry (FC/FACS) (QC tested)
• Application Notes: FC/FACS: Recommended dilution: 1ug/ml
• Conjugation: Biotin
• Immunogen: Platelets
• Preparation and Storage: Store at 2-8 degree C. Do not freeze. Do not use after expiration date stamped on the label.

Flow Cytometry (FC/FACS)

(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD36 (TR9) Biotin antibody (concentration in sample 0,6 ug/ml, Streptavidin APC).)

Flow Cytometry (FC/FACS)

(Separation of thrombocytes stained anti-human CD36 (TR9) Biotin antibody (concentration in sample 0,6 ug/ml, Streptavidin APC, red-filled) from thrombocytes unstained by primary antibody (Streptavidin APC, black-dashed) in flow cytometry analysis (surface staining).)

Related Product Information for anti-CD36 antibody

CD36 (fatty acid translocase, FAT) is an 88kDa ditopic glycosylated protein that belongs to the class B family of scavenger receptors. CD36 is expressed by most resting marginal zone B cells but not by follicular and B1 B cells, and it is rapidly induced on Follicular B cells in vitro upon TLR and CD40 stimulation. CD36 does not affect the development of B cells, but modulates both primary and secondary antibody response. Similarly to glucose transporter GLUT4, CD36 is translocated from intracellular pools to the plasma membrane following cell stimulation by insulin. In mouse, CD36 is responsible for gustatory perception of long-chain fatty acids.

References

*Zamora C, Cantó E, Nieto JC, Ortiz MA, Diaz-Torné C, Diaz-Lopez C, Llobet JM, Juarez C, Vidal S: Functional Application (FUNC)tional consequences of platelet binding to T lymphocytes in inflammation. J Leukoc Biol. 2013 Sep;94(3):521-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/23801652,*Bertrand G, Renac V, Lefaix MC, Nivet C, Trudel E, Richard L: Neonatal Intracranial Hemorrhage with a Dramatic Outcome Due to Maternal Anti CD36 Antibodies. Reports 2019, 2(1), 7; doi:10.3390/reports2010007, URL: https://www.mdpi.com/2571-841X/2/1/7,*Berre S, Gaudin R, Cunha de Alencar B, Desdouits M, Chabaud M, Naffakh N, Rabaza-Gairi M, Gobert FX, Jouve M, Benaroch P: CD36-specific antibodies block release of HIV-1 from infected primary macrophages and its transmission to T cells. J Exp Med. 2013 Nov 18;210(12):2523-38., URL: https://www.ncbi.nlm.nih.gov/pubmed/24145510
*Gaillard D, Laugerette F, Darcel N, El-Yassimi A, Passilly-Degrace P, Hichami A, Akhtar Khan N, Montmayeur JP, Besnard P: The gustatory pathway is involved in CD36-mediated orosensory perception of long-chain fatty acids in the mouse. FASEB J. 2007 Dec 27, URL: https://www.ncbi.nlm.nih.gov/pubmed/18162488ordin,*van Oort MM, van Doorn JM, Bonen A, Glatz JF, van der Horst DJ, Rodenburg KW, Luiken JJ: Insulin-induced translocation of CD36 to the plasma membrane is reversible and shows similarity to that of GLUT4. Biochim Biophys Acta. 2007 Dec 15, URL: https://www.ncbi.nlm.nih.gov/pubmed/18167317ordin,*Won WJ, Bachmann MF, Kearney JF: CD36 Is Differentially Expressed on B Cell Subsets during Development and in Responses to Antigen. J Immunol. 2008 Jan 1;180(1):230-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/18097024ordin

NCBI and Uniprot Product Information

• NCBI GI #: 115982
• NCBI GeneID: 948
• UniProt Accession #: P16671; Q13966; Q16093; Q8TCV7; Q9BPZ8; Q9BQC2; Q9BZM8; Q9BZN3; D9IX66; D9IX67; D9IX68; D9IX69
• Molecular Weight: 472
• NCBI Official Full Name: Platelet glycoprotein 4
• NCBI Official Synonym Full Names: CD36 molecule (thrombospondin receptor)
• NCBI Official Symbol: CD36
• NCBI Official Synonym Symbols: FAT; GP4; GP3B; GPIV; CHDS7; PASIV; SCARB3; BDPLT10
• NCBI Protein Information: platelet glycoprotein 4; GPIIIB; PAS IV; PAS-4 protein; glycoprotein IIIb; cluster determinant 36; fatty acid translocase; platelet glycoprotein IV; scavenger receptor class B, member 3; leukocyte differentiation antigen CD36; CD36 antigen (collagen type
• UniProt Protein Name: Platelet glycoprotein 4
• Protein Family: Platelet glycoprotein
• UniProt Gene Name: CD36
• UniProt Synonym Gene Names: GP3B; GP4; FAT; GPIIIB; GPIV
• UniProt Entry Name: CD36_HUMAN

NCBI Description

The protein encoded by this gene is the fourth major glycoprotein of the platelet surface and serves as a receptor for thrombospondin in platelets and various cell lines. Since thrombospondins are widely distributed proteins involved in a variety of adhesive processes, this protein may have important functions as a cell adhesion molecule. It binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. It directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes and it binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport. Mutations in this gene cause platelet glycoprotein deficiency. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Feb 2014]

Uniprot Description

CD36: Seems to have numerous potential physiological functions. Binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. May function as a cell adhesion molecule. Directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes. Binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport. Receptor for thombospondins, THBS1 AND THBS2, mediating their antiangiogenic effects. Defects in CD36 are the cause of platelet glycoprotein IV deficiency (PG4D)[MIM:608404]; also known as CD36 deficiency. Platelet glycoprotein IV deficiency can be divided into 2 subgroups. The type I phenotype is characterized by platelets and monocytes/macrophages exhibiting complete CD36 deficiency. The type II phenotype lacks the surface expression of CD36 in platelets, but expression in monocytes/macrophages is near normal. Genetic variations in CD36 are associated with susceptibility to coronary heart disease type 7 (CHDS7). Belongs to the CD36 family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 7q11.2

Cellular Component: Golgi apparatus; extracellular space; platelet alpha granule membrane; cell surface; membrane; integral to plasma membrane; plasma membrane; phagocytic vesicle; lipid raft; external side of plasma membrane

Molecular Function: low-density lipoprotein receptor activity; transforming growth factor beta binding; low-density lipoprotein binding; lipid binding; high-density lipoprotein binding

Biological Process: positive regulation of blood coagulation; cGMP-mediated signaling; positive regulation of interleukin-12 production; phagocytosis, recognition; negative regulation of transcription from RNA polymerase II promoter; cellular lipid metabolic process; negative regulation of transcription factor import into nucleus; low density lipoprotein mediated signaling; sequestering of lipid; antigen processing and presentation of peptide antigen via MHC class I; cell surface receptor linked signal transduction; platelet degranulation; positive regulation of MAPKKK cascade; antigen processing and presentation of exogenous peptide antigen via MHC class I; positive regulation of cell-matrix adhesion; cell adhesion; toll-like receptor 4 signaling pathway; receptor-mediated endocytosis; platelet activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; cholesterol transport; positive regulation of interleukin-6 production; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; positive regulation of tumor necrosis factor production; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; defense response to Gram-positive bacterium; positive regulation of peptidyl-tyrosine phosphorylation; lipoprotein transport; toll-like receptor signaling pathway; innate immune response; lipid metabolic process; blood coagulation; positive regulation of phagocytosis, engulfment; plasma membrane long-chain fatty acid transport; nitric oxide mediated signal transduction; apoptotic cell clearance

Disease: Platelet Glycoprotein Iv Deficiency; Coronary Heart Disease, Susceptibility To, 7; Malaria, Susceptibility To