Testing Data
(Representative blot from a previous lot. HeLa acid extract (lane 1 and lanes 3 through 7) and recombinant histone H3 (lane 2) were resolved by electrophoresis, transferred to nitrocellulose and probed with MBS616986 H3 (Lys4) (0.5ug/ml; lanes 1 and 2) or probed with antibody at the same concentration pre-incubated with 1uM histone H3 peptides with the following modifications: Lane 3: monomethyl-lysine 4 Lane 4: dimethyl-lysine 4 Lane 5: trimethyl-lysine 4 Lane 6: monomethyl-lysine 9 Lane 7: monomethyl-lysine 27 Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates monomethyl-histone H3 (Lys4), (~17kD).)
Rabbit anti-Human Histone H3 Polyclonal Antibody | anti-HIST3H3 antibody
Histone H3, monomethyl (Lys4) (PE)
Gene Names
HIST3H3; H3t; H3.4; H3/g; H3FT
Reactivity
Human
Applications
Western Blot, Chromatin Immunoprecipitation, Immunoprecipitation, Immunocytochemistry
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
Histone H3; Polyclonal Antibody; monomethyl (Lys4) (PE); anti-HIST3H3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human monomethyl-histone H3 (Lys4), Mr 17kD. Specificity was confirmed by the ability of 1uM of the immunizing peptide to completely abolish detection of histone H3 in immunoblot analysis of HeLa acid extracts. No competition was observed with peptides containing dimethyl-lysine 4, trimethyl-lysine 4, mono-methyllysine 4 or monomethyl-lysine 9 or 27.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-HIST3H3 antibody
Western Blot (WB), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC)
Application Notes
Applications are based on unconjugated antibody.
WB: 0.5-2ug/ul. Detects methylated histone H3 in acid extracted proteins from HeLa cells. HeLa acid extract and recombinant histone H3 were resolved by electrophoresis, transferred to nitrocellulose and probed with.5ug/ml). Proteins were visualized using a goat anti-rabbit secondary antidody conjugated to HRP and a chemiluminescence detection system.
WB: 0.5-2ug/ul. Detects methylated histone H3 in acid extracted proteins from HeLa cells. HeLa acid extract and recombinant histone H3 were resolved by electrophoresis, transferred to nitrocellulose and probed with.5ug/ml). Proteins were visualized using a goat anti-rabbit secondary antidody conjugated to HRP and a chemiluminescence detection system.
Immunogen
Synthetic peptide containing the sequence ART[meK]..., in which meK corresponds to monomethyl-lysine at residue 4 of human histone H3. (KLH coupled)
Conjugate
PE
Positive Control
HeLa cell extract
Note
Preservative Free
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Testing Data
(Representative blot from a previous lot. HeLa acid extract (lane 1 and lanes 3 through 7) and recombinant histone H3 (lane 2) were resolved by electrophoresis, transferred to nitrocellulose and probed with MBS616986 H3 (Lys4) (0.5ug/ml; lanes 1 and 2) or probed with antibody at the same concentration pre-incubated with 1uM histone H3 peptides with the following modifications: Lane 3: monomethyl-lysine 4 Lane 4: dimethyl-lysine 4 Lane 5: trimethyl-lysine 4 Lane 6: monomethyl-lysine 9 Lane 7: monomethyl-lysine 27 Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates monomethyl-histone H3 (Lys4), (~17kD).)
Testing Data
(Representative blot from a previous lot. HeLa acid extract (lane 1 and lanes 3 through 7) and recombinant histone H3 (lane 2) were resolved by electrophoresis, transferred to nitrocellulose and probed with MBS616986 H3 (Lys4) (0.5ug/ml; lanes 1 and 2) or probed with antibody at the same concentration pre-incubated with 1uM histone H3 peptides with the following modifications: Lane 3: monomethyl-lysine 4 Lane 4: dimethyl-lysine 4 Lane 5: trimethyl-lysine 4 Lane 6: monomethyl-lysine 9 Lane 7: monomethyl-lysine 27 Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates monomethyl-histone H3 (Lys4), (~17kD).)
Product Categories/Family for anti-HIST3H3 antibody
References
1. Kohlmaier, A., et al., PLoS Biol. 2: E171, 2004 (Epub). 2. Strahl, B.D., et al., Proc. Natl. Acad. Sci. USA 96: 14967-14972, 1999.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
136
NCBI Official Full Name
histone H3.1t
NCBI Official Synonym Full Names
histone cluster 3, H3
NCBI Official Symbol
HIST3H3
NCBI Official Synonym Symbols
H3t; H3.4; H3/g; H3FT
NCBI Protein Information
histone H3.1t; H3/t; histone 3, H3; H3 histone family, member T
UniProt Protein Name
Histone H3.1t
Protein Family
UniProt Gene Name
HIST3H3
UniProt Synonym Gene Names
H3FT; H3/t; H3t
UniProt Entry Name
H31T_HUMAN
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Product Notes
The HIST3H3 hist3h3 (Catalog #AAA6480591) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H3, monomethyl (Lys4) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC). Applications are based on unconjugated antibody. WB: 0.5-2ug/ul. Detects methylated histone H3 in acid extracted proteins from HeLa cells. HeLa acid extract and recombinant histone H3 were resolved by electrophoresis, transferred to nitrocellulose and probed with.5ug/ml). Proteins were visualized using a goat anti-rabbit secondary antidody conjugated to HRP and a chemiluminescence detection system. Researchers should empirically determine the suitability of the HIST3H3 hist3h3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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