Western Blot (WB)
(WB: Acid extracts from untreated (Lane 1) and VP16-treated (Lane 2) HL-60 cell lysates were probed with MBS620928)
Rabbit anti-Human Histone H2B Polyclonal Antibody
Histone H2B, phosphorylated (Ser14), aa6-16
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry
Purity
SerumSerum
Synonyms
Histone H2B; Polyclonal Antibody; phosphorylated (Ser14); aa6-16; Anti -Histone H2B; anti-Histone H2B antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes hunan phospho-Histone H2B (Ser14), Mr 15kD. Specific for the phosphorylated peptide and does not crossreact with the unphosphorylated counterpart.
Purity/Purification
Serum
Serum
Serum
Form/Format
Supplied as a liquid, 0.05% sodium azide, 30% glycerol.
Applicable Applications for anti-Histone H2B antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Application Notes
Suitable for use in ELISA, Western Blot, Immunocytochemistry and Immunohistochemistry.
Dilution: Western Blot: 1:1000-1:10,000. Detected phospho-Histone H2B in HL-60 cells treated with 20ug/ml VP16 for 18 hours. A 1:250-1:2000 dilution also detected phospho-Histone H2B acid extracted proteins from colcemid-arrested HeLa cells.
Immunocytochemistry: 1:5000 selectively immunostained pycnotic apoptotic HL-60 cells and TUNEL positive HeLa cells.
Immunohistochemistry: 1:5000 immunostained deparaffinated Xenopus tail sections.
Note: Use of this antibody at dilutions which are too low will result in non-specific detection of Histone H2B and other unknown proteins in immunoblots of acid extracts from untreated cells.
Dilution: Western Blot: 1:1000-1:10,000. Detected phospho-Histone H2B in HL-60 cells treated with 20ug/ml VP16 for 18 hours. A 1:250-1:2000 dilution also detected phospho-Histone H2B acid extracted proteins from colcemid-arrested HeLa cells.
Immunocytochemistry: 1:5000 selectively immunostained pycnotic apoptotic HL-60 cells and TUNEL positive HeLa cells.
Immunohistochemistry: 1:5000 immunostained deparaffinated Xenopus tail sections.
Note: Use of this antibody at dilutions which are too low will result in non-specific detection of Histone H2B and other unknown proteins in immunoblots of acid extracts from untreated cells.
Immunogen
Synthetic peptide corresponding to aa 6-16, C-SAPAPKKG[pS]KK of human histone H2B (KLH).
Species Sequence Homology
Broad
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(WB: Acid extracts from untreated (Lane 1) and VP16-treated (Lane 2) HL-60 cell lysates were probed with MBS620928)
Western Blot (WB)
(WB: Acid extracts from untreated (Lane 1) and VP16-treated (Lane 2) HL-60 cell lysates were probed with MBS620928)
Immunocytochemistry (ICC)
(ICC: Detection of H2B phosphorylated at serine 14 in HL-60 cells stimulated to undergo apoptosis by treatment with VP-16. Cells were stained with phospho-Histone H2B (Ser14). (Courtesy of Dr. David Allis, The Rockefeller University).)
Immunocytochemistry (ICC)
(ICC: Detection of H2B phosphorylated at serine 14 in HL-60 cells stimulated to undergo apoptosis by treatment with VP-16. Cells were stained with phospho-Histone H2B (Ser14). (Courtesy of Dr. David Allis, The Rockefeller University).)
Related Product Information for anti-Histone H2B antibody
Histone 2B is the core component of nucleosome. It is involved in nucleosome wrapping and the compaction of DNA into chromatin, limiting DNA accessibility. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated via a complex system of nucleosome remodelling and post-translational modification of histones, also called histone code.
Product Categories/Family for anti-Histone H2B antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The Histone H2B (Catalog #AAA620928) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H2B, phosphorylated (Ser14), aa6-16 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H2B can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC). Suitable for use in ELISA, Western Blot, Immunocytochemistry and Immunohistochemistry. Dilution: Western Blot: 1:1000-1:10,000. Detected phospho-Histone H2B in HL-60 cells treated with 20ug/ml VP16 for 18 hours. A 1:250-1:2000 dilution also detected phospho-Histone H2B acid extracted proteins from colcemid-arrested HeLa cells. Immunocytochemistry: 1:5000 selectively immunostained pycnotic apoptotic HL-60 cells and TUNEL positive HeLa cells. Immunohistochemistry: 1:5000 immunostained deparaffinated Xenopus tail sections. Note: Use of this antibody at dilutions which are too low will result in non-specific detection of Histone H2B and other unknown proteins in immunoblots of acid extracts from untreated cells. Researchers should empirically determine the suitability of the Histone H2B for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H2B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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