Western Blot (WB)
(Western blot analysis of Hela cell lysate using 1 ug/ml Rabbit Anti-Histone 2B Polyclonal Antibody The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.)
Rabbit anti-Human Histone H2B Polyclonal Antibody | anti-H2B antibody
Histone H2B
Gene Names
H2B; MGC159573
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
Histone H2B; Polyclonal Antibody; Anti -Histone H2B; anti-H2B antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes endogenous levels of human histone 2B protein.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a lyophilized powder in PBS, pH 7.4, 0.02% sodium azide.
Applicable Applications for anti-H2B antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 0.05-0.2ug/ml
Western Blot: 0.5-1ug/ml
Dilution: ELISA: 0.05-0.2ug/ml
Western Blot: 0.5-1ug/ml
Immunogen
Synthetic peptide (KLH). derived from the carboxyl terminal of human histone 2B (Swiss Prot: Q99879).
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western blot analysis of Hela cell lysate using 1 ug/ml Rabbit Anti-Histone 2B Polyclonal Antibody The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.)
Western Blot (WB)
(Western blot analysis of Hela cell lysate using 1 ug/ml Rabbit Anti-Histone 2B Polyclonal Antibody The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.)
Related Product Information for anti-H2B antibody
Histone 2B is the core component of nucleosome. It is involved in nucleosome wrapping and the compaction of DNA into chromatin, limiting DNA accessibility. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated via a complex system of nucleosome remodelling and post-translational modification of histones, also called histone code.
Product Categories/Family for anti-H2B antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
histone H2B
NCBI Official Symbol
H2B
NCBI Official Synonym Symbols
MGC159573
NCBI Protein Information
histone H2B
Protein Family
Similar Products
Product Notes
The H2B (Catalog #AAA619913) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H2B reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H2B can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: ELISA: 0.05-0.2ug/ml Western Blot: 0.5-1ug/ml. Researchers should empirically determine the suitability of the H2B for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H2B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
