Western Blot (WB)
(All lanes : Anti-Histone H2B Antibody at 1:1000 dilutionLane 1: A549 whole cell lysatesLane 2: WiDr whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 14 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human, mouse Histone H2B Polyclonal Antibody | anti-H2BFS antibody
Histone H2B Antibody
Western Blot (WB)
(All lanes : Anti-Histone H2B Antibody at 1:1000 dilutionLane 1: A549 whole cell lysatesLane 2: WiDr whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 14 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-Histone H2B Antibody at 1:1000 dilutionLane 1: A549 whole cell lysatesLane 2: WiDr whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 14 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Hattori M.,et al.Nature 405:311-319(2000).
Kim H.S.,et al.J. Immunol. 168:2356-2364(2002).
Frohm M.,et al.Eur. J. Biochem. 237:86-92(1996).
Tollin M.,et al.Peptides 24:523-530(2003).
NCBI and Uniprot Product Information
Uniprot Description
H2BFS: a core component of the nucleoosome. The nucleosome, a basic organizational unit of chromosomal DNA, is octrameric, consisting of two molecules each of histones H2B, H2A, H3, H4. The octamer wraps approximately 147 bp of DNA. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 21q22.3
Cellular Component: extracellular space; nucleosome; nucleus
Molecular Function: DNA binding; protein heterodimerization activity
Biological Process: defense response to Gram-positive bacterium; substantia nigra development; innate immune response in mucosa; antibacterial humoral response
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Product Notes
The H2BFS h2bfs (Catalog #AAA9208565) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-60. The Histone H2B Antibody reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H2B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC), Immunohistochemistry (IHC)-Paraffin. WB~~ 1:1000. Researchers should empirically determine the suitability of the H2BFS h2bfs for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H2B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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