HER3/ERBB3 Active Protein | ERBB3 active protein

HER3/ERBB3 Protein, Rat, Recombinant (hFc Tag)

Cat. Num. AAA8123354

• Gene Names: Erbb3; nuc-ErbB3
• Purity: >80% as determined by SDS-PAGE
• Synonyms: HER3/ERBB3; HER3/ERBB3 Protein; Rat; Recombinant (hFc Tag); Rat HER3/ErbB3 Protein (Fc Tag); erb-b2 receptor tyrosine kinase 3; ERBB3 active protein

• Host: HEK293 Cells
• Purity/Purification: >80% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-His641

• Species: Rat
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ser 20
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the rat ERBB3 (Q62799)(Met1-His641) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: Measured by its binding ability in a functional ELISA.; 1. Immobilized rat ERBB3-Fc at 10ug/ml (100uL/well) can bind biotinylated human NRG1 (isoform Beta1) with a linear range of 0.018-0.125ug/ml.; 2. Immobilized rat ERBB3-Fc at 10ug/ml (100uL/well) can bind biotinylated human NRG1 (aa 2-246)-Fc with a linear range of 0.018-0.25ug/ml.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for ERBB3 active protein

Background: ErbB3, also known as Her3(human epidermal growth factor receptor3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain., and therefore can not mediate the intracellular signal transduction through protein phosphorylation. However, its heterodimer with ErbB2 or other EGFR members responsible for tyrosine phosphorylation forms a receptor complex with high affinity, and initiates the related pathway which lead to cell proliferation or differentiation. ErbB3 has been shown to implicated in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.

Product Categories/Family for ERBB3 active protein

Cancer Drug Targets Receptor Tyrosine Kinases (RTKs)

References

Kraus M.H., et al.,(1989), Isolation and characterization of ERBB3, a third member of the ERBB/epidermal growth factor receptor family: evidence for overexpression in a subset of human mammary tumors. Proc. Natl. Acad. Sci. U.S.A. 86:9193-9197.
Plowman G.D., et al., (1990), Molecular cloning and expression of an additional epidermal growth factor receptor-related gene.Proc. Natl. Acad. Sci. U.S.A. 87:4905-4909.
Katoh M., et al.,(1993), c-erbB3 gene encodes secreted as well as transmembrane receptor tyrosine kinase.Biochem. Biophys. Res. Commun. 192:1189-1197.

NCBI and Uniprot Product Information

• NCBI GI #: 17432904
• NCBI GeneID: 29496
• NCBI Accession #: NP_058914.2
• NCBI GenBank Nucleotide #: NM_017218.3
• UniProt Accession #: Q62799; Q62955
• Molecular Weight: 147,546 Da
• NCBI Official Full Name: receptor tyrosine-protein kinase erbB-3
• NCBI Official Synonym Full Names: v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 3
• NCBI Official Symbol: Erbb3
• NCBI Official Synonym Symbols: nuc-ErbB3
• NCBI Protein Information: receptor tyrosine-protein kinase erbB-3; c-erbB3; c-erbB-3; avian erythroblastosis oncogene B 3; proto-oncogene-like protein c-ErbB-3; v-erb-b2 erythroblastic leukemia viral oncogene homolog 3
• UniProt Protein Name: Receptor tyrosine-protein kinase erbB-3
• Protein Family: Receptor tyrosine-protein kinase
• UniProt Gene Name: Erbb3
• UniProt Entry Name: ERBB3_RAT

NCBI Description

acts as a tyrosine kinase receptor for neuregulin; may play a role in axonal regeneration in response to injury [RGD, Feb 2006]

Uniprot Description

HER3: a receptor tyrosine kinase of the EGFR family. Binds and is activated by neuregulins and NTAK. Can form homodimers or ErbB-2/ErbB-3 heterodimers. Kinase domain lacks activity but heterodimerizes with other EGFRs to transduce growth signals. May be required for HER2 activity. Elevated expression in breast and other tumors is indicative of poor outcome. A secreted form is expressed in metastatic prostate cancer Two alternatively spliced isoforms have been described.

Protein type: Protein kinase, TK; EC 2.7.10.1; Oncoprotein; Protein kinase, tyrosine (receptor); Kinase, protein; Membrane protein, integral; TK group; EGFR family

Cellular Component: postsynaptic membrane; extracellular space; basolateral plasma membrane; apical plasma membrane; integral to membrane; nucleus; lateral plasma membrane; receptor complex

Molecular Function: identical protein binding; protein heterodimerization activity; protein-tyrosine kinase activity; growth factor binding; protein tyrosine kinase activator activity; receptor signaling protein tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; ATP binding

Biological Process: circadian rhythm; epidermal growth factor receptor signaling pathway; response to drug; tongue development; mammary gland involution; skeletal muscle development; phosphoinositide 3-kinase cascade; peptidyl-tyrosine phosphorylation; cranial nerve development; negative regulation of cell adhesion; signal transduction; regulation of cell proliferation; negative regulation of signal transduction; peripheral nervous system development; Schwann cell differentiation; odontogenesis; positive regulation of glucose import; neuron apoptosis; axonogenesis; cellular response to insulin stimulus; response to wounding; negative regulation of secretion; negative regulation of neuron apoptosis; transmembrane receptor protein tyrosine kinase signaling pathway