Rabbit Histone H2A.Z/H2AFZ Polyclonal Antibody | anti-H2AFZ antibody

Anti-Histone H2A.Z/H2AFZ Antibody, Rabbit Polyclonal

Cat. Num. AAA8101341

• Gene Names: H2AFZ; H2AZ; H2A.z; H2A/z; H2A.Z-1
• Reactivity: Human; Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: Protein A & Antigen Affinity
• Synonyms: Histone H2A.Z/H2AFZ; Polyclonal Antibody; Anti-Histone H2A.Z/H2AFZ Antibody; Rabbit Polyclonal; H2AFZ Antibody; Rabbit PAb; Antigen Affinity Purified; H2A histone family member Z; Anti-H2A.z Antibody; Anti-H2A.Z-1 Antibody; Anti-H2A/z Antibody; Anti-H2AZ Antibody; anti-H2AFZ antibody

• Host: Rabbit
• Reactivity: Human; Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human Histone H2A.Z/H2AFZ
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-H2AFZ antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; ICC/IF: 1:1000-1:5000
• Immunogen: A synthetic peptide corresponding to the N-terminus of the Human H2AFZ
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human H2AFZ, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-H2AFZ rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLane B: NIH3T3 Whole Cell LysateLane C: HepG2 Whole Cell LysateLane D: Raji Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:13 kDaObserved band size:15 kDa)

Immunofluorescence (IF)

(Immunofluorescence staining of H2AFZ in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti- H2AFZ polyclonal antibody (1:5000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red).Positive staining was localized to nucleus.)

Related Product Information for anti-H2AFZ antibody

H2AZ1 (H2A.Z Variant Histone 1, also known as H2AFZ) is a Protein Coding gene. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. H2AFZ gene may confer a risk for schizophrenia and contribute to the impairment of executive function in Han Chinese patients with schizophrenia. H2AFZ gene may be a susceptibility factor in male subjects with schizophrenia, and that modification of the H2AFZ signaling pathway warrants further study in terms of the pathophysiology of schizophrenia. H2AFZ is widely expressed in the bone marrow, lymph node, and other tissues. Diseases associated with H2AZ1 include Testicular Spermatocytic Seminoma and Floating-Harbor Syndrome.

NCBI and Uniprot Product Information

• NCBI GI #: 4504255
• NCBI GeneID: 3015
• NCBI Accession #: NP_002097.1
• NCBI GenBank Nucleotide #: NM_002106.3
• UniProt Accession #: P0C0S5; P17317; Q6I9U0; B2RD56
• Molecular Weight: 13,553 Da
• NCBI Official Full Name: histone H2A.Z
• NCBI Official Synonym Full Names: H2A histone family, member Z
• NCBI Official Symbol: H2AFZ
• NCBI Official Synonym Symbols: H2AZ; H2A.z; H2A/z; H2A.Z-1
• NCBI Protein Information: histone H2A.Z; H2AZ histone
• UniProt Protein Name: Histone H2A.Z
• UniProt Gene Name: H2AFZ
• UniProt Synonym Gene Names: H2AZ; H2A/z
• UniProt Entry Name: H2AZ_HUMAN

NCBI Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality. [provided by RefSeq, Jul 2008]

Uniprot Description

H2AZ: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. H2A or its variant H2AFZ forms an heterodimer with H2B. H2AFZ interacts with INCENP. Belongs to the histone H2A family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 4q24

Cellular Component: Barr body; nucleosome; nuclear heterochromatin; nucleus

Molecular Function: protein binding; nucleosomal DNA binding; chromatin DNA binding; protein heterodimerization activity

Biological Process: positive regulation of transcription from RNA polymerase II promoter