Sheep Histone, Core Polyclonal Antibody | anti-Histone antibody
Histone, Core
Reactivity
Bovine, Human
Applications
ELISA, Western Blot, Immunoprecipitation
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
Histone; Core; Polyclonal Antibody; Anti -Histone; anti-Histone antibody
Host
Sheep
Reactivity
Bovine, Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes all bovine histones at ~15kD. Species Crossreactivity: human
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.4. No preservatives added.
Applicable Applications for anti-Histone antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
Application Notes
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applilcations have not been tested.
Dilution: Immunoprecipitation: 1:20
Western Blot: 1:200
Dilution: Immunoprecipitation: 1:20
Western Blot: 1:200
Immunogen
Intact calf Histone core complex to rRNA
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for anti-Histone antibody
Autoantibodies to histone antigens have been described in patients with idiopathic and drug-induced SLE, rheumatoid arthritis and other conditions. The presence of autoantibodies to histones are frequently found in several rheumatic disorders. In one study, the predominant responses to histones in SLE sera were to H1, H2b, and H3. Marked elevations of binding occurred to H1 and H2b in 33% of patients, while 25% showed higher binding to H3. The same study showed the highest anti-histone reactivity to be in patients with rheumatoid arthritis with vasculitis, while the highest reactivity in SLE sera was in those patients with a history of photosensitivity. In diploid eukaryotic cells, the chromatin fibers are about 20nm in diameter. They consist of two major components in equal amounts, DNA and basic proteins called histones. Histones are believed to be regularly arranged in the deep grove of the DNA helix. The recurring positive charges of the histones form electrostatic associations with the negatively charged phosphate groups of DNA making the DNA more stable and flexible. This allows for the supercoiling of the chromatin fibers.
Product Categories/Family for anti-Histone antibody
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
Lysine Rich (H1, f1): ~21,500
Slightly Lysine Rich (H2a, f2a2): 14,004
Slightly Lysine Rich (H2b, f2b): 13,774
Arginine Rich (H3, f3): 15,324
Arginine Rich (H4, f2a1): 11,282
Slightly Lysine Rich (H2a, f2a2): 14,004
Slightly Lysine Rich (H2b, f2b): 13,774
Arginine Rich (H3, f3): 15,324
Arginine Rich (H4, f2a1): 11,282
NCBI Official Full Name
Histone
UniProt Protein Name
Histone protein
UniProt Gene Name
Histone
UniProt Entry Name
Q5ZP39_9VIRU
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Product Notes
The Histone, Core histone (Catalog #AAA620903) is an Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. The Histone, Core reacts with Bovine, Human and may cross-react with other species as described in the data sheet. AAA Biotech's Histone, Core can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP). Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applilcations have not been tested. Dilution: Immunoprecipitation: 1:20 Western Blot: 1:200. Researchers should empirically determine the suitability of the Histone, Core histone for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone, Core, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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