Goat HISTIDINE TAG Polyclonal Antibody
GOAT ANTI HISTIDINE TAG:Biotin
Applications
ELISA, Western Blot
Synonyms
HISTIDINE TAG; Polyclonal Antibody; GOAT ANTI HISTIDINE TAG:Biotin; anti-HISTIDINE TAG antibody
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Specificity
Goat anti-Histidine tag antibody recognizes the motif H-H-H-H-H-H and is therfore of value in detecting proteins containing histicine tags. Goat anti-Histidine tag antibody may not detect all N-Terminal (NT) histidine tagged proteins. Goat anti-Histidine tag antibody has not been shown to be suitable for use on cytospins.
Form/Format
Purified IgG conjugated to Biotin - liquid; Buffer Solution: Phosphate buffered saline
Concentration
IgG concentration 1.0mg/ml (varies by lot)
Applicable Applications for anti-HISTIDINE TAG antibody
ELISA (EIA), Western Blot (WB)
Application Notes
EIA: 1:10000-1:100000
WB: 1:10000-1:100000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is receommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
WB: 1:10000-1:100000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is receommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
0.2% Bovine Serum Albumin
0.2% Bovine Serum Albumin
Immunogen
Keyhole limpet haemocyanin (KLH) conjugated H-H-H-H-H-H
Antiserum Preparation
Antiserum to Histidine Tag was raised by repeated immunization of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Target Species
Synthetic Peptide
Preparation and Storage
Store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Related Product Information for anti-HISTIDINE TAG antibody
Goat anti-Histidine tag antibody recognizes the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. Goat anti-Histidine tag antibody may not detect all N-Terminal (NT) histidine tagged proteins. Goat anti-Histidine tag antibody has been shown to be suitable for use on cytospins.
Similar Products
Product Notes
The HISTIDINE TAG (Catalog #AAA224190) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's HISTIDINE TAG can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). EIA: 1:10000-1:100000 WB: 1:10000-1:100000 Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is receommended that the user titrates the product for use in their own system using appropriate negative/positive controls. Researchers should empirically determine the suitability of the HISTIDINE TAG for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HISTIDINE TAG, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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