Mouse DAZ1 Monoclonal Antibody | anti-DAZ1 antibody

DAZ1 (Deleted in Azoospermia 1, DAZ, SPGY) (PE)

Cat. Num. AAA6184149

• Gene Names: RAF1; NS5; CRAF; Raf-1; c-Raf; CMD1NN
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified
• Synonyms: DAZ1; Monoclonal Antibody; DAZ1 (Deleted in Azoospermia 1; DAZ; SPGY) (PE); Deleted in Azoospermia 1; SPGY; anti-DAZ1 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 3000
• Specificity: Recognizes DAZ1.
• Purity/Purification: Purified
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Sequence Length: 648

• Applicable Applications for anti-DAZ1 antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: DAZ1 (AAH18119, 21aa-120aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26kD.
• Conjugate: PE
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Testing Data

(Immunoperoxidase of monoclonal antibody to DAZ1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 1 ug/ml])

Immunohistochemistry (IHC)

(Immunoperoxidase of monoclonal antibody to DAZ1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 1 ug/ml])

Western Blot (WB)

(DAZ1 monoclonal antibody (M06), clone 3E3. Western Blot analysis of DAZ1 expression in Daoy.)

Testing Data

(Detection limit for recombinant GST tagged DAZ1 is approximately 0.1ng/ml as a capture antibody.)

Related Product Information for anti-DAZ1 antibody

This gene is a member of the DAZ gene family and is a candidate for the human Y-chromosomal azoospermia factor (AZF). Its expression is restricted to premeiotic germ cells, particularly in spermatogonia. It encodes an RNA-binding protein that is important for spermatogenesis. Four copies of this gene are found on chromosome Y within palindromic duplications; one pair of genes is part of the P2 palindrome and the second pair is part of the P1 palindrome. Each gene contains a 2.4 kb repeat including a 72-bp exon, called the DAZ repeat; the number of DAZ repeats is variable and there are several variations in the sequence of the DAZ repeat. Each copy of the gene also contains a 10.8 kb region that may be amplified; this region includes five exons that encode an RNA recognition motif (RRM) domain. This gene contains three copies of the 10.8 kb repeat. However, no transcripts containing three copies of the RRM domain have been described; thus the RefSeq for this gene contains only two RRM domains. [provided by RefSeq]

Product Categories/Family for anti-DAZ1 antibody

Antibodies; Binding Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 17390263
• NCBI GeneID: 5894
• NCBI Official Full Name: V-raf-1 murine leukemia viral oncogene homolog 1
• NCBI Official Synonym Full Names: Raf-1 proto-oncogene, serine/threonine kinase
• NCBI Official Symbol: RAF1
• NCBI Official Synonym Symbols: NS5; CRAF; Raf-1; c-Raf; CMD1NN
• NCBI Protein Information: RAF proto-oncogene serine/threonine-protein kinase
• Protein Family: Deleted in azoospermia protein

NCBI Description

This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq, Jul 2008]