Western Blot (WB)
(Western blot analysis of HIPK3 using HepG2 whole cell lysates)
Rabbit anti-Human HIPK3 Polyclonal Antibody | anti-HIPK3 antibody
HIPK3 Antibody
Gene Names
HIPK3; PKY; YAK1; DYRK6; FIST3
Reactivity
Human
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
HIPK3; Polyclonal Antibody; HIPK3 Antibody; Androgen receptor interacting nuclear protein kinase; Androgen receptor-interacting nuclear protein kinase; ANPK; DYRK6; Fas interacting serine/threonine protein kinase; Fas-interacting serine/threonine-protein kinase; FIST; FIST3; Hipk3; HIPK3_HUMAN; homeodomain interacting protein kinase 3; Homeodomain-interacting protein kinase 3; Homolog of protein kinase YAK1; PKY; RP24 297H17.5; YAK1; anti-HIPK3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
HIPK3 antibody detects endogenous levels of total HIPK3
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
1194
Applicable Applications for anti-HIPK3 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:1000-3000
Immunogen
A synthesized peptide derived from human HIPK3
Subcellular Location
Cytoplasm. Nucleus.
Tissue Specificity
Overexpressed in multidrug resistant cells. Highly expressed in heart and skeletal muscle, and at lower levels in placenta, pancreas, brain, spleen, prostate, thymus, testis, small intestine, colon and leukocytes. Not found in liver and lung.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Similarity
Pig (100%), Bovine (100%), Horse (82%), Sheep (100%), Rabbit (92%), Dog (100%), Chicken (100%), Xenopus (91%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of HIPK3 using HepG2 whole cell lysates)
Western Blot (WB)
(Western blot analysis of HIPK3 using HepG2 whole cell lysates)
Related Product Information for anti-HIPK3 antibody
Function: Serine/threonine-protein kinase involved in transcription regulation, apoptosis and steroidogenic gene expression. Phosphorylates JUN and RUNX2. Seems to negatively regulate apoptosis by promoting FADD phosphorylation. Enhances androgen receptor-mediated transcription. May act as a transcriptional corepressor for NK homeodomain transcription factors. The phosphorylation of NR5A1 activates SF1 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. In osteoblasts, supports transcription activation: phosphorylates RUNX2 that synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE).
Subunit Structure: Interacts with Nkx1-2. Interacts with FAS and DAXX. Probably part of a complex consisting of HIPK3, FAS and FADD. Interacts with and stabilizes ligand-bound androgen receptor (AR) (By similarity). Interacts with UBL1/SUMO-1. Binds to NR5A1/SF1, SPEN/MINT and RUNX2.
Post-translational Modifications: Autophosphorylated, but autophosphorylation is not required for catalytic activity. May be sumoylated.
Similarity: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. HIPK subfamily.
Subunit Structure: Interacts with Nkx1-2. Interacts with FAS and DAXX. Probably part of a complex consisting of HIPK3, FAS and FADD. Interacts with and stabilizes ligand-bound androgen receptor (AR) (By similarity). Interacts with UBL1/SUMO-1. Binds to NR5A1/SF1, SPEN/MINT and RUNX2.
Post-translational Modifications: Autophosphorylated, but autophosphorylation is not required for catalytic activity. May be sumoylated.
Similarity: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. HIPK subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 134 kDa
Predicted: 134 kDa
Predicted: 134 kDa
NCBI Official Full Name
homeodomain-interacting protein kinase 3 isoform 2
NCBI Official Synonym Full Names
homeodomain interacting protein kinase 3
NCBI Official Symbol
HIPK3
NCBI Official Synonym Symbols
PKY; YAK1; DYRK6; FIST3
NCBI Protein Information
homeodomain-interacting protein kinase 3
UniProt Protein Name
Homeodomain-interacting protein kinase 3
Protein Family
UniProt Gene Name
HIPK3
UniProt Synonym Gene Names
DYRK6; FIST3; PKY; ANPK; FIST
Uniprot Description
Serine/threonine-protein kinase involved in transcription regulation, apoptosis and steroidogenic gene expression. Phosphorylates JUN and RUNX2. Seems to negatively regulate apoptosis by promoting FADD phosphorylation. Enhances androgen receptor-mediated transcription. May act as a transcriptional corepressor for NK homeodomain transcription factors. The phosphorylation of NR5A1 activates SF1 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. In osteoblasts, supports transcription activation: phosphorylates RUNX2 that synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE).
Research Articles on HIPK3
Similar Products
Product Notes
The HIPK3 hipk3 (Catalog #AAA9602938) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HIPK3 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HIPK3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000-3000. Researchers should empirically determine the suitability of the HIPK3 hipk3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HIPK3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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