Immunofluorescence (IF)
(Fluorescent image of HUVEC cell stained with HOXD8 Antibody (C-term).HUVEC cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HOXD8 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C).HOXD8 immunoreactivity is localized to Nucleus significantly. )
Rabbit anti-Human HOXD8 Polyclonal Antibody | anti-HOXD8 antibody
HOXD8, CT (HOXD8, HOX4E, Homeobox protein Hox-D8, Homeobox protein Hox-4E, Homeobox protein Hox-5.4) (AP)
Gene Names
HOXD8; HOX4; HOX4E; HOX5.4
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
HOXD8; Polyclonal Antibody; CT (HOXD8; HOX4E; Homeobox protein Hox-D8; Homeobox protein Hox-4E; Homeobox protein Hox-5.4) (AP); Homeobox protein Hox-5.4; anti-HOXD8 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Sequence Length
290
Applicable Applications for anti-HOXD8 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
HOXD8 antibody is generated from rabbits immunized with a KLH conjugated synthetic between 242-270 amino acids selected from the C-terminal region of human HOXD8.
Conjugate
AP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Immunofluorescence (IF)
(Fluorescent image of HUVEC cell stained with HOXD8 Antibody (C-term).HUVEC cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HOXD8 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C).HOXD8 immunoreactivity is localized to Nucleus significantly. )
Immunofluorescence (IF)
(Fluorescent image of HUVEC cell stained with HOXD8 Antibody (C-term).HUVEC cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HOXD8 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C).HOXD8 immunoreactivity is localized to Nucleus significantly. )
Western Blot (WB)
(HOXD8 Antibody (C-term) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the HOXD8 antibody detected the HOXD8 protein (arrow).)
Western Blot (WB)
(HOXD8 Antibody (C-term) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the HOXD8 antibody detected the HOXD8 protein (arrow).)
Related Product Information for anti-HOXD8 antibody
This gene belongs to the homeobox family of genes. The homeobox genes encode a highly conserved family of transcription factors that play an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters, HOXA, HOXB, HOXC and HOXD, located on different chromosomes, consisting of 9 to 11 genes arranged in tandem. This gene is one of several homeobox HOXD genes located in a cluster on chromosome 2. Deletions that remove the entire HOXD gene cluster or the 5' end of this cluster have been associated with severe limb and genital abnormalities. In addition to effects during embryogenesis, this particular gene may also play a role in adult urogenital tract function.
Product Categories/Family for anti-HOXD8 antibody
References
Ester, A.R., et al. Am. J. Med. Genet. A 149A (12), 2745-2752 (2009): Yerges, L.M., et al. J. Bone Miner. Res. 24(12):2039-2049(2009). Harada, K., et al. J. Cell. Sci. 122 (PT 21), 3923-3930 (2009): Goodman, F.R. Am. J. Med. Genet. 112(3):256-265(2002). Kosaki, K., et al. Teratology 65(2):50-62(2002).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
homeobox protein Hox-D8 isoform 1
NCBI Official Synonym Full Names
homeobox D8
NCBI Official Symbol
HOXD8
NCBI Official Synonym Symbols
HOX4; HOX4E; HOX5.4
NCBI Protein Information
homeobox protein Hox-D8
UniProt Protein Name
Homeobox protein Hox-D8
Protein Family
UniProt Gene Name
HOXD8
UniProt Synonym Gene Names
HOX4E
UniProt Entry Name
HXD8_HUMAN
NCBI Description
This gene belongs to the homeobox family of genes. The homeobox genes encode a highly conserved family of transcription factors that play an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters, HOXA, HOXB, HOXC and HOXD, located on different chromosomes, consisting of 9 to 11 genes arranged in tandem. This gene is one of several homeobox HOXD genes located in a cluster on chromosome 2. Deletions that remove the entire HOXD gene cluster or the 5' end of this cluster have been associated with severe limb and genital abnormalities. In addition to effects during embryogenesis, this particular gene may also play a role in adult urogenital tract function. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Dec 2010]
Research Articles on HOXD8
Similar Products
Product Notes
The HOXD8 hoxd8 (Catalog #AAA6308166) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HOXD8, CT (HOXD8, HOX4E, Homeobox protein Hox-D8, Homeobox protein Hox-4E, Homeobox protein Hox-5.4) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HOXD8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the HOXD8 hoxd8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HOXD8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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